Figure 7: Nature Communications

Fig. 7

From: Structural basis of adaptor-mediated protein degradation by the tail-specific PDZ-protease Prc

Fig. 7

Mutational analysis of NlpI-MepS interaction. a Sedimentation velocity profiles of dimeric wild-type sNlpI and monomeric sNlpI-ΔN. b ITC analysis of the interaction of sNlpI-ΔN with sMepS showing no binding. c Sedimentation velocity profiles of sNlpI mutants showing dimer formation. dg ITC analysis of the interaction of sMepS with sNlpI-L38A d, sNlpI-L38C e, sNlpI-R82E f, and sNlpI-A114W g. h and i Effect of nlpI-L38C overexpression in cells of MG1655∆mepM h or MG1655∆nlpI i containing pTrc99A or pCHT93 carrying the WT or mutant nlpI derivatives. Viability assays were done similarly as in Fig. 5c, with or without 0.05 mM IPTG