Figure 5: Nature Communications

Fig. 5

From: Structural basis of adaptor-mediated protein degradation by the tail-specific PDZ-protease Prc

Fig. 5

Mutational analysis of NlpI on Prc interaction. a Pull-down assays of Prc-K477A by wild-type (WT) or various mutants of 6×His-tagged sNlpI. b SDS-PAGE assay monitoring substrate degradation by Prc and sNlpI-QM. c Effects of NlpI mutations in cells of MG1655∆nlpI or MG1655∆mepM containing pTrc99A or pCHT93 carrying the WT or mutant NlpI derivatives. Cultures were grown in LB broth with ampicillin and 5 µl of various dilutions were spotted on indicated plates and grown overnight. TM, triple D113A/E117A/E124A mutant; QM, quadruple D113A/E117A/D120/E124A mutant