Abstract
Semaphorins, specifically type IV, are important regulators of axonal guidance and have been increasingly implicated in poor prognoses in a number of different solid cancers. In conjunction with their cognate PLXNB family receptors, type IV members have been increasingly shown to mediate oncogenic functions necessary for tumor development and malignant spread. In this study, we investigated the role of semaphorin 4C (SEMA4C) in osteosarcoma growth, progression, and metastasis. We investigated the expression and localization of SEMA4C in primary osteosarcoma patient tissues and its tumorigenic functions in these malignancies. We demonstrate that overexpression of SEMA4C promotes properties of cellular transformation, while RNAi knockdown of SEMA4C promotes adhesion and reduces cellular proliferation, colony formation, migration, wound healing, tumor growth, and lung metastasis. These phenotypic changes were accompanied by reductions in activated AKT signaling, G1 cell cycle delay, and decreases in expression of mesenchymal marker genes SNAI1, SNAI2, and TWIST1. Lastly, monoclonal antibody blockade of SEMA4C in vitro mirrored that of the genetic studies. Together, our results indicate a multi-dimensional oncogenic role for SEMA4C in metastatic osteosarcoma and more importantly that SEMA4C has actionable clinical potential.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 50 print issues and online access
$259.00 per year
only $5.18 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Morrow JJ, Khanna C. Osteosarcoma genetics and epigenetics: emerging biology and candidate therapies. Crit Rev Oncogenesis. 2015;20:173–97.
Yan GN, Lv YF, Guo QN. Advances in osteosarcoma stem cell research and opportunities for novel therapeutic targets. Cancer Lett. 2016;370:268–74.
Gianferante DM, Mirabello L, Savage SA. Germline and somatic genetics of osteosarcoma—connecting aetiology, biology and therapy. Nat Rev Endocrinol. 2017;13:480–91.
Rickel K, Fang F, Tao J. Molecular genetics of osteosarcoma. Bone. 2017;102:69–79.
Mirabello L, Troisi RJ, Savage SA. Osteosarcoma incidence and survival rates from 1973 to 2004: data from the Surveillance, Epidemiology, and End Results Program. Cancer. 2009;115:1531–43.
Alto LT, Terman JR. Semaphorins and their signaling mechanisms. Methods Mol Biol. 2017;1493:1–25.
Meyer LA, Fritz J, Pierdant-Mancera M, Bagnard D. Current drug design to target the Semaphorin/Neuropilin/Plexin complexes. Cell Adhes Migr. 2016;10:700–8.
Tamagnone L. Emerging role of semaphorins as major regulatory signals and potential therapeutic targets in cancer. Cancer Cell. 2012;22:145–52.
Worzfeld T, Offermanns S. Semaphorins and plexins as therapeutic targets. Nat Rev Drug Discov. 2014;13:603–21.
Negishi-Koga T, Shinohara M, Komatsu N, Bito H, Kodama T, Friedel RH, et al. Suppression of bone formation by osteoclastic expression of semaphorin 4D. Nat Med. 2011;17:1473–80.
Moriarity BS, Otto GM, Rahrmann EP, Rathe SK, Wolf NK, Weg MT, et al. A sleeping beauty forward genetic screen identifies new genes and pathways driving osteosarcoma development and metastasis. Nat Genet. 2015;47:615–24.
Abarrategi A, Tornin J, Martinez-Cruzado L, Hamilton A, Martinez-Campos E, Rodrigo JP, et al. Osteosarcoma: cells-of-origin, cancer stem cells, and targeted therapies. Stem Cells Int. 2016;2016:3631764.
Swiercz JM, Worzfeld T, Offermanns S. ErbB-2 and met reciprocally regulate cellular signaling via plexin-B1. J Biol Chem. 2008;283:1893–901.
Gurrapu S, Pupo E, Franzolin G, Lanzetti L, Tamagnone L. Sema4C/PlexinB2 signaling controls breast cancer cell growth, hormonal dependence and tumorigenic potential. Cell Death Differ. 2018;25:1259–75.
Lu J, Lin Y, Li F, Ye H, Zhou R, Jin Y, et al. MiR-205 suppresses tumor growth, invasion, and epithelial-mesenchymal transition by targeting SEMA4C in hepatocellular carcinoma. FASEB J. 2018;32:fj201800113R.
Le AP, Huang Y, Pingle SC, Kesari S, Wang H, Yong RL, et al. Plexin-B2 promotes invasive growth of malignant glioma. Oncotarget. 2015;6:7293–304.
Paldy E, Simonetti M, Worzfeld T, Bali KK, Vicuna L, Offermanns S, et al. Semaphorin 4C Plexin-B2 signaling in peripheral sensory neurons is pronociceptive in a model of inflammatory pain. Nat Commun. 2017;8:176.
Xue D, Desjardins M, Kaufman GN, Béland M, Al-Tamemi S, Ahmed E, et al. Semaphorin 4C: A novel component of B-cell polarization in Th2-driven immune responses. Front Immunol. 2016;7:558.
Wei JC, Yang J, Liu D, Wu MF, Qiao L, Wang JN, et al. Tumor-associated lymphatic endothelial cells promote lymphatic metastasis by highly expressing and secreting SEMA4C. Clin Cancer Res. 2017;23:214–24.
Jiang WG, Sanders AJ, Katoh M, Ungefroren H, Gieseler F, Prince M, et al. Tissue invasion and metastasis: Molecular, biological and clinical perspectives. Semin Cancer Biol. 2015;35 Suppl:S244–s275.
Mori S, Chang JT, Andrechek ER, Matsumura N, Baba T, Yao G, et al. Anchorage-independent cell growth signature identifies tumors with metastatic potential. Oncogene. 2009;28:2796–805.
Butti R, Kumar TV, Nimma R, Kundu GC. Impact of semaphorin expression on prognostic characteristics in breast cancer. Breast Cancer. 2018;10:79–88.
GarcÃa Z, Kumar A, Marqués M, Cortés I, Carrera AC. Phosphoinositide 3-kinase controls early and late events in mammalian cell division. EMBO J. 2006;25:655–61.
Smeester BA, Al-Gizawiy M, Beitz AJ. Effects of different electroacupuncture sch eduling regimens on murine bone tumor-induced hyperalgesia: sex differences and role of inflammation. Evid-Bed Complement Altern Med. 2012;2012:671386.
Smeester BA, Al-Gizawiy M, O’Brien EE, Ericson ME, Triemstra JL, Beitz AJ. The effect of electroacupuncture on osteosarcoma tumor growth and metastasis: analysis of different treatment regimens. Evid-Based Complement Altern Med. 2013;2013:387169.
Yang Q, Wang Y, Lu X, Zhao Z, Zhu L, Chen S, et al. MiR-125b regulates epithelial-mesenchymal transition via targeting Sema4C in paclitaxel-resistant breast cancer cells. Oncotarget. 2015;6:3268–79.
Zhou QD, Ning Y, Zeng R, Chen L, Kou P, Xu CO, et al. Erbin interacts with Sema4C and inhibits Sema4C-induced epithelial-mesenchymal transition in HK2 cells. J Huazhong Univ Sci Technol Med Sci = Hua zhong ke ji da xue xue bao Yi xue Ying De wen ban = Huazhong keji daxue xuebao Yixue Yingdewen ban. 2013;33:672–9.
Roche J. The Epithelial-to-Mesenchymal Transition in Cancer. Cancers. 2018;10:1–4.
Yin K, Liao Q, He H, Zhong D. Prognostic value of Twist and E-cadherin in patients with osteosarcoma. Med Oncol. 2012;29:3449–55.
Horvai AE, Roy R, Borys D, O’Donnell RJ. Regulators of skeletal development: a cluster analysis of 206 bone tumors reveals diagnostically useful markers. Mod Pathol. 2012;25:1452–61.
Lee KW, Lee NK, Ham S, Roh TY, Kim SH. Twist1 is essential in maintaining mesenchymal state and tumor-initiating properties in synovial sarcoma. Cancer Lett. 2014;343:62–73.
Choo S, Wang P, Newbury R, Roberts W, Yang J. Reactivation of TWIST1 contributes to Ewing sarcoma metastasis. Pediatr Blood Cancer. 2018;65:1–18.
Leder K, Holland EC, Michor F. The therapeutic implications of plasticity of the cancer stem cell phenotype. PLoS ONE. 2010;5:e14366.
Poleszczuk J, Enderling H. Cancer stem cell plasticity as tumor growth promoter and catalyst of population collapse. Stem Cells Int. 2016;2016:3923527.
Chan CKF, Gulati GS, Sinha R, Tompkins JV, Lopez M, Carter AC, et al. Identification of the human skeletal stem cell. Cell. 2018;175:43–56.e21.
Lamouille S, Xu J, Derynck R. Molecular mechanisms of epithelial-mesenchymal transition. Nat Rev Mol Cell Biol. 2014;15:178–96.
Kumar S, Das A, Sen S. Extracellular matrix density promotes EMT by weakening cell–cell adhesions. Mol Biosyst. 2014;10:838–50.
Ye X, Weinberg RA. Epithelial-mesenchymal plasticity: a central regulator of cancer progression. Trends Cell Biol. 2015;25:675–86.
Meazza C, Scanagatta P. Metastatic osteosarcoma: a challenging multidisciplinary treatment. Expert Rev Anticancer Ther. 2016;16:543–56.
Deng S, Hirschberg A, Worzfeld T, Penachioni JY, Korostylev A, Swiercz JM, et al. Plexin-B2, but not Plexin-B1, critically modulates neuronal migration and patterning of the developing nervous system in vivo. J Neurosci. 2007;27:6333–47.
Clavijo PE, Friedman J, Robbins Y, Moore EC, Smith ES, Zauderer M, et al. Semaphorin4D inhibition improves response to immune checkpoint blockade via attenuation of MDSC recruitment and function. Cancer Immunol Res. 2018;7:282–91.
Evans EE, Jonason AS Jr., Bussler H, Torno S, Veeraraghavan J, Reilly C, et al. Antibody blockade of semaphorin 4D promotes immune infiltration into tumor and enhances response to other immunomodulatory therapies. Cancer Immunol Res. 2015;3:689–701.
Evans EE, Paris M, Smith ES, Zauderer M. Immunomodulation of the tumor microenvironment by neutralization of Semaphorin 4D. Oncoimmunology. 2015;4:e1054599.
Rahrmann EP, Watson AL, Keng VW, Choi K, Moriarity BS, Beckmann DA, et al. Forward genetic screen for malignant peripheral nerve sheath tumor formation identifies new genes and pathways driving tumorigenesis. Nat Genet. 2013;45:756–66.
Marko TA, Shamsan GA, Edwards EN, Hazelton PE, Rathe SK, Cornax I, et al. Slit-Robo GTPase-activating protein 2 as a metastasis suppressor in osteosarcoma. Sci Rep. 2016;6:39059.
Moriarity BS, Rahrmann EP, Beckmann DA, Conboy CB, Watson AL, Carlson DF, et al. Simple and efficient methods for enrichment and isolation of endonuclease modified cells. PLoS ONE. 2014;9:e96114.
Brett ME, Bomberger HE, Doak GR, Price MA, McCarthy JB, Wood DK. In vitro elucidation of the role of pericellular matrix in metastatic extravasation and invasion of breast carcinoma cells. Integr Biol. 2018;10:242–52.
Khanna C, Prehn J, Yeung C, Caylor J, Tsokos M, Helman L. An orthotopic model of murine osteosarcoma with clonally related variants differing in pulmonary metastatic potential. Clin Exp Metastasis. 2000;18:261–71.
Faustino-Rocha A, Oliveira PA, Pinho-Oliveira J, Teixeira-Guedes C, Soares-Maia R, da Costa RG, et al. Estimation of rat mammary tumor volume using caliper and ultrasonography measurements. Lab Anim. 2013;42:217–24.
Acknowledgements
The authors would like to thank Dr. Kyle Williams for helpful discussion throughout the study, Dr. Sterbs for her intoxicating enthusiasm during the preparation of this paper, Dr. Juan Abrahante for statistical advice, and the Clinical and Translational Science Institute (CSTI) Histology and Research Laboratory team members Dr. Colleen Forester and Lori Holm for tissue preparation and histology services. Author BAS is supported by an NIH NIAMS T32 AR050938 Musculoskeletal Training Grant. Author EJP is supported by an NIH NIAID T32 AI997313 Immunology Training Grant. This work was made possible through funding from the Sobiech Osteosarcoma Fund Award, Randy Shaver Cancer and Community Fund, University of Minnesota Foundation, Rein in Sarcoma Foundation, Aflac-AACR Career Development Award, and the Children’s Cancer Research Fund to author BSM Portions of this work were conducted in the Minnesota Nano Center, which is supported by the National Science Foundation through the National Nano Coordinated Infrastructure Network (NNCI) under Award Number ECCS-1542202. SEMA4C RNA expression results in data set #2 were in whole or part based upon data generated by the Therapeutically Applicable Research to Generate Effective Treatments (TARGET) initiative, phs000218, managed by the NCI. The data used are available (dbGaP accession phs000218.v21.p7). Information about TARGET can be found at http://ocg.cancer.gov/programs/target.
Author information
Authors and Affiliations
Contributions
Conception and design: BAS, DAL, BSM. Development and acquisition of data: BAS, NJS, EJP, HEB, GAS, MRC, JJP, GMD, KLB, EPR. Analysis and interpretation: BAS, NJS, DJO, DKW, JBM, DAL, BSM. Writing, review and revisions: BAS, NJS., EJP, HEB, GAS, MRC, JJP, GMD, KLB, EPR, JBM, DJ.O, DKW, DAL, BSM. Study oversight: BAS, DAL, BSM.
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Smeester, B.A., Slipek, N.J., Pomeroy, E.J. et al. SEMA4C is a novel target to limit osteosarcoma growth, progression, and metastasis. Oncogene 39, 1049–1062 (2020). https://doi.org/10.1038/s41388-019-1041-x
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41388-019-1041-x