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Phosphodiesterase 7B/microRNA-200c relationship regulates triple-negative breast cancer cell growth

Oncogene volume 38, pages 1106–1120 (2019)Cite this article

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Abstract

Members of microRNA-200 (miRNA-200) family have a regulatory role in epithelial to mesenchymal transition (EMT) by suppressing Zeb1 and Zeb2 expression. Consistent with its role in suppressing EMT, Hsa-miR-200c-3p (miR-200c), a member of miR-200 family is poorly expressed in mesenchymal-like triple-negative breast cancer (TNBC) cells and ectopic miR-200c expression suppresses cell migration. In this study, we demonstrated that miR-200c potently inhibited TNBC cell growth and tumor development in a mechanism distinct from its ability to downregulate Zeb1 and Zeb2 expression, because silencing them only marginally affected TNBC cell growth. We identified phosphodiesterase 7B (PDE7B) as a bona fide miR-200c target. Importantly, miR-200c-led inhibition in cell growth and tumor development was prevented by forcing PDE7B transgene expression, while knockdown of PDE7B effectively inhibited cell growth. These results suggest that miR-200c inhibits cell growth by targeting PDE7B mRNA. To elucidate mechanism underlying miR-200c/PDE7B regulation of TNBC cell growth, we showed that cAMP concentration was lower in TNBC cells compared with estrogen receptor-positive (ER + ) cells, and that both miR-200c and PDE7B siRNAs were able to increase cAMP concentration in TNBC cells. High level of cellular cAMP has been shown to induce cell cycle arrest and apoptosis in TNBC cells. Our observation that ectopic expression of miR-200c triggered apoptosis indicates that it does so by elevating level of cellular cAMP. Analysis of breast tumor gene expression datasets revealed an inverse association between miR-200c and PDE7B expression. Especially, both low miR-200c and high PDE7B expression were correlated with poor survival of breast cancer patients. Our study supports a critical role of miR-200c/PDE7B relationship in TNBC tumorigenesis.

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Acknowledgements

This work was supported by funding from NSF of China 81773946, 81573673, 81001666 (DZ), NIH CA187152 (SH), and Florida Breast Cancer Foundation (SH).

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Authors and Affiliations

  1. Institute of Interdisciplinary Integrative Medical Research, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China

    Dan-Dan Zhang, Yuan Xu & Shuang Huang

  2. Department of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville, FL, 32608, USA

    Yue Li & Shuang Huang

  3. Department of Statistics, Sungkyunkwan University, Seoul, 03063, South Korea

    Jaejik Kim

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  1. Dan-Dan Zhang
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Zhang, DD., Li, Y., Xu, Y. et al. Phosphodiesterase 7B/microRNA-200c relationship regulates triple-negative breast cancer cell growth. Oncogene 38, 1106–1120 (2019). https://doi.org/10.1038/s41388-018-0499-2

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