Abstract
Elucidating mechanisms by which physical exercise promotes resilience, the brain’s ability to cope with prolonged stress exposure while maintaining normal psychological functioning, is a major research challenge given the high prevalence of stress-related mental disorders, including major depressive disorder. Chronic voluntary wheel running (VWR), a rodent model that mimics aspects of human physical exercise, induces the transcription factor ΔFosB in the nucleus accumbens (NAc), a key reward-related brain area. ΔFosB expression in NAc modulates stress susceptibility. Here, we explored whether VWR induction of NAc ΔFosB promotes resilience to chronic social defeat stress (CSDS). Male young-adult C57BL/6J mice were single housed for up to 21 d with or without running wheels and then subjected to 10 d of CSDS. Stress-exposed sedentary mice developed a depressive-like state, characterized by anhedonia and social avoidance, whereas stress-exposed mice that had been wheel running showed resilience. Functional inhibition of NAc ΔFosB during VWR, by viral-mediated overexpression of a transcriptionally inactive JunD mutant, reinstated susceptibility to CSDS. Within the NAc, VWR induction of ΔFosB was CREB-dependent, associated with altered dendritic morphology, and medium spiny neuron (MSN) subtype specific in the NAc core and shell subregions. Finally, when mice performed VWR following the onset of CSDS-induced social avoidance, VWR normalized such behavior. These data indicate that VWR promoted resilience to CSDS, and suggest that sustained induction of ΔFosB in the NAc underlies, at least in part, the stress resilience mediated by VWR. These findings provide a potential framework for the development of treatments for stress-associated mental illnesses based on physical exercise.
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Introduction
Major depressive disorder is a common psychiatric condition that results in an enormous social and financial burden on modern society [1, 2]. Although some people develop depressive episodes following acute or chronic episodes of stress, others successfully cope with adversity. Such resilience is associated with the development of behavioral, molecular, and psychological adaptations to stress [3, 4]. Despite recent advances, the neurophysiological processes underlying resilience are still incompletely understood. Full understanding of these processes could offer a crucial dimension for the development of novel therapeutic treatments that prevent stress-related disorders.
ΔFosB, a Fos family member with unique protein stability, heterodimerizes with Jun family proteins to form activator protein-1 transcription factor complexes that regulate the transcription of hundreds of genes [5, 6]. A large literature has demonstrated that ΔFosB in the nucleus accumbens (NAc), a key reward-related brain area, is strongly implicated in neuronal adaptations to drugs of abuse and stress [7,8,9]. Within the NAc, 90–95% of all neurons are projecting medium spiny neurons (MSNs), which are present in approximately equal populations based on their selective expression of dopamine receptor D1 or D2, as well as other genes [10, 11]. These two MSN subtypes, hereafter called D1-MSNs or D2-MSNs, play distinct and often opposing roles in depressive- and addictive-like behavior [12,13,14].
Chronic social defeat stress (CSDS) is an ethologically valid rodent model that induces long-term physiological and behavioral depressive-like phenotypes [15]. Overexpression of ΔFosB specifically in the NAc promoted resilience to CSDS, whereas NAc-specific overexpression of ΔcJun, a transcriptionally inactive truncated cJun mutant that antagonizes ΔFosB activity, promoted susceptibility to CSDS [16]. Intriguingly, although mice exposed to CSDS develop behavioral and metabolic disturbances, a substantial part does not and are therefore termed ‘resilient’ [17]. Molecular follow-up of these cohorts revealed that high levels of ΔFosB in NAc D1-MSNs are associated with stress resilience, whereas high levels of ΔFosB in NAc D2-MSNs are associated with stress susceptibility [18].
Physical exercise has beneficial effects on stress-related mental disorders [19,20,21,22,23,24,25,26], indicating tremendous and low-cost clinical potential. Despite these profound beneficial effects, the underlying neurobiology remains unclear [22]. Voluntary wheel running (VWR), a rodent model that mimics aspects of human physical exercise training, can be used for the preclinical etiological and mechanistic examination of resilience conferred by this behavioral intervention [22]. In rats and mice, 4–6 weeks of VWR increase ΔFosB expression in the NAc [27,28,29,30]. Prior indirect analysis in rats using dynorphin and enkephalin expression (markers of D1- and D2-MSNs, respectively) suggested that VWR induced ΔFosB preferentially in NAc D1-MSNs [30], yet the functional consequences of this accumulation has not been directly investigated to date.
Here, we aimed to examine whether VWR promotes resilience to CSDS, and if NAc ΔFosB is involved. Presence of a depressive-like state was determined based on social avoidance and anhedonia, behavioral reward-related deficits that are controlled by the NAc, associated with depression and which can be objectively measured in rodents [31, 15]. Young-adult male C57BL/6J mice were housed for up to 21 d with or without running wheels and then subjected to 10 d of CSDS. NAc-specific viral-mediated overexpression of ΔJunD, a transcriptionally inactive truncated JunD mutant that antagonizes ΔFosB activity [16], was then utilized to directly test the functional consequences of VWR induction of NAc ΔFosB on stress-related behaviors. We also investigated if VWR after the onset of CSDS-induced social avoidance was able to reverse such aberrant behavior. Finally, several aspects of VWR-induced adaptations in the NAc, including MSN subtype-specific ΔFosB induction, upstream signaling cascades, and dendritic spine morphology, were investigated.
Materials and methods
Animals
All experiments were done in accordance with the policies set out by the institutional animal care and use committee (IACUC) at the Joslin Diabetes Center. C57BL/6J mice (http://jaxmice.jax.org/strain/000664.html) were group housed and acclimated to the Joslin Diabetes Center animal facility for 7 d and housed individually at the start of experiments. Drd1a-TdTomato mice (http://jaxmice.jax.org/strain/016204.html) were bred in-house to wild-type C57BL/6J mice (http://jaxmice.jax.org/strain/000664.html) to generate Drd1a-TdTomato+/- mice. Experimental groups were body weight matched at the start of the experiments. Animals were maintained at 23–25 °C on an artificial 12-h light/dark cycle (lights on from 06:30) with ad libitum access to water and pelleted low-fat chow diet (9F 5020 Lab Diet, 23% protein, 55% carbohydrate, and 22% fat, 3.56 kcal/g, PharmaServ Inc.). Male mice were used for all studies.
Voluntary wheel running
VWR was performed as previously described [32]. In short, after acclimatization, 10-week old mice were housed without (sedentary) or with free (VWR) access to a running wheel (24 cm diameter, 8 cm wide; Nalgene, Rochester, NY) for the indicated experimental time. Wheel revolutions were measured daily or at indicated times using odometers. Because cage enrichment can modulate ΔFosB in the NAc [33], mice housed with a blocked running wheel served as an additional control group, where indicated.
Metabolic measurements
Body weight and available food were measured at the start and end of indicated VWR durations. Water intake was measured during the indicated time using two custom-made non-leaking drinking pipettes, both containing water.
Immunoblotting
For all immunoblotting studies, running wheels remained active for the indicated time and food was removed at the onset of the dark phase. Mice were sacrificed 3–4 h into the dark phase by cervical dislocation, and brains were immediately isolated and snap-frozen in isopentane on dry ice. A separate cohort of mice was sacrificed during the light phase after 14 d of VWR. Isolated brains were subsequently emerged in Optimal Cutting Compound (Tissue-Tek) and cooled before being cut on a freezing microtome (Leica Microsystems, model VT1000S) until onset of the NAc (AP + 1.98 relative to bregma) [34]. NAc samples were bilaterally dissected using a dissection needle (ø 1 mm) and sonicated on ice in 80 μl protein extraction buffer containing phosphatase inhibitor mix 1 (Roche). Samples were centrifuged at 15,000 × g for 25 min following sonication, and supernatant containing whole-cell fractions was collected and processed for protein concentration determination using Bradford protein assays. For western blotting, 30 μg of protein/sample were run on a 10% Criterion acrylamide gel from Bio-Rad, transferred to a nitrocellulose membrane, blocked with 5% bovine serum albumin (BSA), and incubated overnight using primary antibodies against FosB (#2251; 1:1000; Cell Signaling Technology), CREB (#9197; 1:1000; Cell Signaling Technology), pCREB (Ser133) (#9198; 1:1000; Cell Signaling Technology), SRF (1:1000; Santa Cruz Biotechnology), pSRF (Ser103) (#4261; 1:1000; Cell Signaling Technology), and GAPDH (#2118; 1:1000; Cell Signaling Technology). Antibody-bound proteins were visualized on film using chemiluminescence detection reagents (Perkin-Elmer Life Sciences). Protein bands were scanned and quantitated by densitometry using ImageJ software (National Institutes of Health).
Brain perfusion and single label indirect immunofluorescence
After 14 d of VWR, running wheels were blocked for 24 h, resulting in the degradation of any residual full-length FosB protein such that all remaining immunoreactivity reflects ΔFosB [35]. Mice were anesthetized with an overdose of pentobarbital (5 mg/ml saline; 90 mg/kg i.p.) and perfused transcardially with 10 ml 1× phosphate-buffered saline (PBS) for 2 min, followed by 20 ml Histochoice fixative (Amresco) for 4 min. Perfused brains were post-fixed in Histochoice for 48 h at 4 °C, daily transferred to 5%, 15%, and 30% sucrose in PBS at 4 °C, cryopreserved on dry ice in Optimal Cutting Compound (Tissue-Tek), and sliced into 10-µm coronal sections using a freezing microtome (Leica Microsystems, model VT1000S) and mounted. Slides were washed with 1× PBS before being blocked by incubation in 1× PBS with 5% normal goat serum and 0.3% Triton X-100. Rabbit anti-FosB antibody (1:200; #2251, Cell Signaling Technology), diluted in 1× PBS with 0.3% Triton X-100 and 0.1% BSA, was applied to the sections for overnight incubation at 4 °C. Sections were then incubated in Alexa Fluor 488 goat anti-rabbit IgG (1:1000; Life Technologies) at room temperature for 30 min. Slides were cover-slipped with Prolong Gold Antifade reagent containing 4,6-diamidino-2-phenylindole (DAPI; Cell Signaling Technology).
Imaging and cell counting
Immunofluorescence was imaged on a Leica DM 4000B confocal microscope. Cell counting was performed using ImageJ software (National Institutes of Health). Images sampling bregma + 1.4 till + 1.0 of the NAc core and shell subregions and the dorsal striatum were taken from two or three brain sections/animal. The NAc core and shell subregions were differentiated using corresponding diagrams from the Paxinos mouse brain atlas [34]. For the ΔFosB and TdTomato co-staining experiment, approximately 150–300 total DAPI cells were counted per region of interest (ROI) per mouse, and then the number of TdTomato+, TdTomato+: ΔFosB+, TdTomato–, and TdTomato–: ΔFosB+ cells were counted in each ROI. If necessary, ΔFosB+ cell numbers were manually corrected for background staining of white fiber tissue. The total number of TdTomato– cells was corrected for the presence of non-MSN cells. Data were quantified as follows: (TdTomato+: ΔFosB+ neurons × 100%)/(total TdTomato+ neurons) and (TdTomato–: ΔFosB+ neurons × 95%)/(total TdTomato– neurons).
CSDS and behavioral evaluations
CSDS was performed as previously described [32] (see Supplementary Material and Methods for full details).
Two-bottle sucrose preference test
Sucrose preference tests were performed as previously described [32] (see Supplementary Material and Methods for full details).
Open-field test
Open-field tests were performed as previously described [32] (see Supplementary Material and Methods for full details).
Stereotaxic surgery
Overexpression of mCREB or ΔJunD specifically in the NAc was achieved by injecting an adeno-associated virus (rAAV2) vector expressing mCREB, or ΔJunD [16, 36]. The ΔJunD virus preparation was spiked with rAAV2-GFP (1:10) to allow for assessment of injection site. Control mice were injected with a rAAV2-GFP. Intra-NAc injections were performed as previously described [37] (see Supplementary Material and Methods for full details).
Dendritic spine analysis
Dendritic spine analysis was performed as previously described [38] (see Supplementary Material and Methods for full details).
Statistical analysis
All data are presented as mean ± SEM. Two group comparisons were performed by two-tailed Student's t-test. Assessment of effects in experiments involving several conditions was performed using one-, two-, or three-way analysis of variance (ANOVA), with repeated measures where applicable, followed, when appropriate, by Tukey HSD post hoc tests, adjusting for multiple comparisons. For all cases, a P-value < 0.05 was considered significant. See figure legends for statistical details of individual experiments, including statistical tests used, t, P, F-values, and number of subjects or samples tested.
Results
VWR prior to CSDS prevents development of social avoidance and anhedonia
To test if VWR prevents the development of depression-like behaviors during CSDS, we used a rodent model in which 10-week-old male C57BL/6J mice were single housed without (sedentary) or with (VWR) free access to running wheels for 21 d before being subjected to 10 d of CSDS (Fig. 1a). By the end of the second week, mice with wheel access were consistently running approximately 7 km per day (Fig. 1b). VWR lowered body weight gain, increased food and water intake, but did not affect basal sucrose preference (Figures S1a-e).
After 21 d of VWR or sedentary housing, mice were further subdivided in mice that remained unexposed to aggressor mice (i.e., undefeated controls) or mice that were subjected to CSDS (i.e., defeated), a paradigm that in our hands reliably induces depression-like behavior [16, 17, 32]. In brief, social defeat was induced by daily 10-min exposure to an aggressor for 10 d, with the remainder of each day spent in sensory contact. Control mice were handled daily and spent each day in sensory contact with another experimental mouse (Fig. 1a). On day 11, 24 h after the last social defeat episode, all defeated and control mice underwent a social interaction test, where mice are placed in an open-field set-up containing a small cage containing an unfamiliar mouse (Figure S2a). The amount of time spent interacting with or avoiding the unfamiliar mouse serves as a readout for social avoidance, which correlates well with several other depression-related behavioral abnormalities [17]. Two-way ANOVA analysis revealed a main effect of CSDS on social behavior (Figs. 1c, d). Additional analysis, using an a posteriori t-test, revealed that defeated VWR mice showed a reduced propensity to develop social avoidance compared with defeated sedentary mice (Figs. 1c, d), suggesting that VWR prior to CSDS decreases susceptibility to subsequent social defeat. In support of this, analysis of social interaction ratios, a measure used to segregate resilient and susceptible populations [17], revealed that VWR increased the proportion of resilient mice (Figs. 1e, f). Differences in social behavior between the defeated sedentary and defeated VWR mice could not be explained by differences in locomotor activity during the social interaction test (Figure S2b), nor by differences in stress exposure, as indicated by similar changes in body weight, minor wounds sustained and stress-induced polydipsia during night 11 (Figures S2c-e). We then used an open-field test to measure locomotor activity and the amount of time spent in the center or periphery of an open-field area, as a readout for anxiety-like behavior. All defeated mice showed reduced locomotor activity and increased anxiety-like behavior, as indicated by less center zone entries, compared with undefeated controls (Figures S2f and g). Sucrose preference is measured by simultaneously offering two bottles, one with a sugar dilution and one with normal water. A decrease in sucrose preference is another measure of depression-like behavior, which is indicative of anhedonia [15]. Defeated sedentary mice showed a significant decrease in sucrose preference compared with pre-social defeat levels, whereas both defeated VWR mice and undefeated controls did not (Fig. 1f).
Spatial and temporal aspects of VWR induction of ΔFosB in the NAc
To assess the spatial induction of ΔFosB in the striatum, wild-type mice were housed without or with free access to running wheels for 14 d (Figures S3a and b). Quantitative immunohistochemistry was subsequently used to identify ΔFosB-positive neurons in striatal regions (Fig. 2a). VWR mice had more ΔFosB-positive neurons than sedentary mice in the NAc core, NAc shell, and the dorsomedial striatum, whereas ΔFosB-positive neurons in the dorsolateral striatum were relatively less abundant and similar between experimental groups (Figs. 2b, c).
To study the temporal dynamics of ΔFosB induction in the NAc during VWR, we performed western blot analysis on NAc dissections containing both core and shell subregions of the NAc (Figure S4a). Using this approach, we confirmed that VWR promotes accumulation of ΔFosB in the NAc (Fig. 2d). Furthermore, cage enrichment controls (i.e., mice housed with a blocked running wheel) showed no significant induction of ΔFosB in the NAc compared with sedentary mice, suggesting that it was the VWR, not the presence of a running wheel, which resulted in accumulation of ΔFosB in the NAc (Fig. 2d). Finally, we found that 14 d, or longer, of VWR, but not 7 d, resulted in significant accumulation of ΔFosB in the NAc compared with sedentary controls (Figures S4b-d).
Prior indirect analysis in rats, using dynorphin expression as a marker for D1-MSNs and enkephalin expression as a marker for D2-MSNs, suggested that VWR induces ΔFosB preferentially in D1-MSNs of the NAc core [30], although this has heretofore not been examined directly. To examine this directly, we made use of a mouse model in which TdTomato expression is driven by the Drd1a promoter [39], with the presence of TdTomato (TdTomato+) defining D1-MSNs and the absence of TdTomato (TdTomato–) likely defining D2-MSNs [40]. Cell-specific induction of ΔFosB in the NAc was assessed following 14 d of VWR and 1 day of wheel blockade (Figures S3a and c). Immunohistochemical analysis determined that VWR increased ΔFosB in both TdTomato+ and TdTomato– MSNs of the NAc core (Figs. 2e, f). In contrast, VWR induction of ΔFosB predominantly occurred in TdTomato+ MSNs of the NAc shell (Figs. 2e, f).
CREB is required for VWR induction of ΔFosB in NAc
We next investigated upstream signaling pathways mediating VWR induction of ΔFosB in NAc. Based on studies using chronic cocaine treatment [37], we investigated if serum response factor (SRF) and cAMP response element-binding protein (CREB) might be involved in VWR induction of NAc ΔFosB. Western blot analysis of whole NAc dissections revealed similar phosphorylation of CREB at serine 133 (Ser133) and of SRF at serine 103 (Ser103) in sedentary and VWR mice sacrificed during the light phase, when mice are resting (Figures S5a-c). In contrast, when sedentary and VWR mice were sacrificed during the early dark phase, when mice are active, VWR was associated with increased CREB (Ser133) phosphorylation compared with sedentary controls. In addition, CREB (Ser133) phosphorylation correlated strongly with the amount of VWR performed just before brains were isolated (Figures S5d and f). There was no effect of VWR on SRF (Ser103) phosphorylation (Figures S5d, g, and h).
To directly test if CREB signaling is necessary for VWR induction of NAc ΔFosB, we virally overexpressed a dominant-negative mutant protein that functionally inhibits endogenous CREB (mCREB) [6] or the fluorescent reporter green fluorescent protein (GFP), as a control, in the NAc of sedentary and VWR mice (Figures S5i-l). Overexpression of mCREB in the NAc prevented VWR induction of ΔFosB in the NAc (Fig. 2g).
VWR modulates dendritic morphology of NAc MSNs
Dendritic spines can be divided into three distinct functional subclasses that include immature stubby spines, which have a clear spine head but lack a neck, and neck-bearing immature thin and mature mushroom spines where the size of the head region (larger in mushroom spines) is among the primary distinguishing features of these spine types [41]. Short-term overexpression of ΔFosB in NAc increases immature, predominantly stubby, spines on MSNs [42]. In addition, ΔFosB expression is associated with the formation and/or the maintenance of dendritic spines on NAc MSNs after chronic cocaine treatment [43]. How VWR modulates NAc spine morphogenesis in mice remains unknown. To assess this, we infused HSV-GFP into the NAc of VWR mice and sedentary controls. After 21 d of VWR, total spine density in NAc MSNs showed an increased trend in VWR mice compared with sedentary controls (Figs. 3a, b). This increase was driven by a significant induction of immature thin spines (Figs. 3c, d).
Transcriptional blockade of NAc ΔFosB during VWR reinstates susceptibility to CSDS
To test the functional consequences of VWR induction of NAc ΔFosB, we virally overexpressed a dominant-negative JunD mutant protein (ΔJunD) that antagonizes ΔFosB function [16], or GFP as a control, in the NAc (Fig. 4a; S6a and b). Transcriptional silencing of ΔFosB in NAc has no effect on baseline measures of locomotor activity and anxiety-like behavior [16]. Wild-type C57BL/6J mice overexpressing ΔJunD or GFP in the NAc were given free access to running wheels for 21 d before being subjected to 10 d of CSDS (Fig. 4a). Sedentary undefeated and defeated C57BL/6J mice served as additional controls (Fig. 4a). Mice overexpressing GFP or ΔJunD specifically in the NAc demonstrated normal VWR behavior, although daily and cumulative running distances were slightly lower in mice overexpressing ΔJunD (Figure S6c). Overexpression of ΔJunD in the NAc did not affect metabolic responding to VWR or preference for a 1% sucrose solution (Figures S6d-h).
During the social interaction test on day 11, and confirming our previous results, VWR mice overexpressing GFP in the NAc showed reduced propensity to develop social avoidance and anhedonia compared with defeated sedentary mice (Figs. 4b, c). In contrast, mice with NAc-specific transcriptional blockade of ΔFosB during VWR developed social avoidance and anhedonia, indicative of reinstated susceptibility to CSDS (Figs. 4b, c). Differences in social behavior between the defeated mice overexpressing either GFP or ΔJunD in the NAc could not be explained by altered locomotor activity during the social interaction test (Figure S7a), nor by differences in exposure to social defeat stress, as indicated by similar changes in body weight, minor wounds sustained, and stress-induced polydipsia during night 11 (Figures S7b-d). All defeated mice showed hypoactivity and increased anxiety-like behavior compared with undefeated controls in the open-field test (Figures S7e and f).
VWR following CSDS ameliorates CSDS-induced social avoidance
CSDS-induced social avoidance is maintained over time [17]. Therefore, we assessed if VWR following CSDS was able to restore aberrant social behavior in susceptible mice (Fig. 5a). When examined 24 h after the last social defeat (“day 11”), 10 out of 20 defeated mice were labeled “susceptible” based on their social interaction scores (data not shown). This percentage is representative for C57BL/6J mice [17]. As expected, susceptible mice showed social avoidance compared with undefeated controls (Fig. 5b). Subsequently, a subset of the susceptible mice was given free access to running wheels after day 11, whereas the remainder of susceptible mice remained sedentary. When examined 42 d after the last defeat (“day 53”), susceptible mice that had been running showed social behavior similar to the level of undefeated controls, whereas sedentary susceptible mice still displayed social avoidance (Figs. 5b, c). Differences in social behavior between the susceptible mice housed either sedentary or with running wheels could not be explained by altered locomotor activity during the social interaction tests (Fig. 5d).
Discussion
Physical exercise has well-documented beneficial effects on stress-related mental disorders, but the underlying mechanisms remain incompletely understood. Here, we demonstrate that 21 days of VWR prevents the development of social avoidance and anhedonia during CSDS, whereas anxiety-related behaviors measured in the open-field test remained unchanged. Furthermore, using a combination of behavioral, molecular, and viral gene transfer approaches, we demonstrate that VWR induction of ΔFosB in the NAc subregions occurs in specific MSN populations, VWR is associated with changes in MSN dendritic morphology, and that functional blockade of NAc ΔFosB during VWR reinstated susceptibility to CSDS. These data suggest that VWR induction of NAc ΔFosB contributes to the pro-resilient effects of VWR observed in this study (Fig. 5e). Finally, 42 days of VWR ameliorated CSDS-induced social avoidance behavior, indicative of antidepressant potential of VWR.
Although both the NAc, as well as ΔFosB have been heavily implicated in stress responses and mood disorders [7], our study is the first attempt to directly link NAc ΔFosB to the pro-resilient effects of VWR. Mechanistic studies in rodents, investigating the antidepressant potential of VWR, have previously indicated the involvement of several brain nuclei, most notably the hippocampus and the basolateral amygdala [22, 44,45,46]. Our findings now add the NAc to the brain circuitry involved in the beneficial effects of VWR on stress-related behavior. This is in line with the role of the ventral striatal motor-limbic interface that integrates emotional information from several brain regions, including the basolateral amygdala, and translates such information into behaviorally relevant outputs [9].
VWR induction of ΔFosB occurred equally in D1- and D2-MSNs in the NAc core, yet was selective for D1-MSNs in the NAc shell. This ΔFosB induction pattern in the NAc subregions resembles the induction pattern observed in response to 10-min optogenetic activation of ventral tegmental area neurons on 5 consecutive days [18], suggesting that activation of dopaminergic neurons in the ventral tegmental area during VWR, and subsequent activation of dopamine receptors in the NAc, is an important factor that drives accumulation of NAc ΔFosB. Although activation of dopaminergic ventral tegmental area neurons during VWR has to date not been measured directly, observations that the ventral tegmental area can modulate VWR behavior [47, 48], and that VWR induces plasticity along the ventral tegmental area–striatum axis [27, 29], support this possibility.
The VWR induction pattern of NAc ΔFosB observed in this study differs from the induction pattern observed after 28 d of juvenile environmental enrichment or 10 d of a 10% sugar dilution, naturally rewarding conditions that both show equal induction of ΔFosB in D1- and D2-MSNs in the NAc subregions [18]. It should, however, be noted that VWR transiently increases plasma corticosterone levels during the first 3 weeks of VWR and is associated with profound adaptations along the hypothalamic–pituitary–adrenal axis [22, 49]. Thus, VWR should be considered a physiological stressor, especially during the initial weeks of VWR. Furthermore, VWR animals and their sedentary controls are commonly single housed to accurately measure VWR behavior and its metabolic consequences. Prolonged isolation, 8 weeks or longer, can increase susceptibility to stress in rodents [16, 50]. In this study, we did not observe anhedonia in any of our experiments following the 3 weeks of single housing prior to CSDS. This suggests that this duration is not sufficient to induce a depressive-like state. However, we cannot rule out that the social isolation primed susceptibility to CSDS, an effect that could be prevented by VWR. In this light, it will be interesting to investigate if VWR retains its pro-resilient capacity in group-housed animals.
Following CSDS, resilient mice show selective induction of ΔFosB in NAc D1-MSNs, whereas susceptible mice show selective induction of ΔFosB in D2-MSNs [18]. Thus, equal induction in D1-MSNs and D2-MSNs in NAc core, but selective induction in D1-MSNs in NAc shell, after 14 d of VWR likely results from a temporal interplay between reward- and stress-related brain circuitries. Further work is needed to identify the role of the two MSN subtypes, the different striatal subregions, as well as temporal NAc neurotransmitter dynamics in mediating the beneficial effects of VWR on stress-related behavior. Potential neurotransmitters involved are brain-derived neurotrophic factor, dopamine, and lactate, as all of these factors are centrally modulated by physical exercise and involved in the modulation of depressive-like behavior [22, 51,52,53,54,55].
What are the potential mechanisms underlying the regulation of stress resilience by VWR? We observed a trend for an increase in total dendritic spine density on NAc MSNs following VWR, resulting from an increase in thin, but not stubby or mushroom, spines. Although we have not directly investigated the functional consequences of these spine type-specific alterations in NAc MSNs following VWR, ΔFosB is known to modulate synaptic properties of NAc MSNs and subsequent reward-related behavior [42, 56, 57].
Susceptible, but not resilient, mice demonstrate an increase in immature stubby spines on NAc MSNs in response to CSDS [58, 59]. Similarly, short-term overexpression of ΔFosB in NAc predominantly increases stubby spines on D1-MSNs [42]. However, these studies did not reveal an increase in NAc thin spines in resilient mice or following short-term overexpression of ΔFosB. In contrast, early (i.e., 4 h) withdrawal from cocaine is associated with an increase in thin, but not stubby or mushroom, spines in the NAc shell, but not the core, subregion [60]. These observations suggest that the specific increase in thin spines observed in this study could result from the time when the VWR mice were euthanized, that is, during the light phase when mice are resting (i.e., voluntary short-term withdrawal from VWR). Similar to our observations in mice, a recent study in female Wistar rats demonstrated that 3 weeks of VWR is associated with increased total spine density, and a relative increase and decrease in thin and stubby spines, respectively [61]. Collectively, these studies provide strong rationale to investigate how VWR alters synaptic maturation and function in the NAc, and to identify the functional consequences of altered VWR-associated NAc MSN spine formation on stress resilience. Such studies should take into account the duration of and/or withdrawal from VWR as well as functional differences between the NAc subregions and MSN subtypes.
Full understanding of the neurobiological adaptations during physical exercise is critical in order to develop rational and optimal treatment strategies for patients suffering from stress-related disorders. Furthermore, such understanding can aid in the prevention of stress-related disorders in healthy subjects. Our identification of ΔFosB in the NAc as a likely contributor to the greater stress resilience following VWR provides new insight into the nature of VWR-mediated adaptations in the NAc. The possible relevance of our results to human psychiatry is suggested by the observations that high physical activity modulates dopaminergic neurotransmission in the striatum of human subjects [62, 63]. This neuronal pathway may therefore represent a target for the development of therapeutic treatments for stress-associated mental illnesses based on physical exercise.
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Acknowledgements
We are grateful to Christopher Cahill (Joslin Diabetes Center microscopy core) and Dr. Peter Hamilton (Mount Sinai) for assistance with imaging studies, and members of the Goodyear lab for helpful comments throughout the course of this project. This work was supported by National Institutes of Health (NIH) grants R01-DK-101043 and R01-DK-099511 to LJG; NIH grant 5P30-DK-36836 to the Diabetes Research Center, Joslin Diabetes Center; and a National Institute of Mental Health grant R01-MH051399 to EJN.; JDM was supported by an American Diabetes Association Mentor-based Award to LJG (7-12-MN-22).
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Mul, J.D., Soto, M., Cahill, M.E. et al. Voluntary wheel running promotes resilience to chronic social defeat stress in mice: a role for nucleus accumbens ΔFosB. Neuropsychopharmacol 43, 1934–1942 (2018). https://doi.org/10.1038/s41386-018-0103-z
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DOI: https://doi.org/10.1038/s41386-018-0103-z
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