Abstract
Invasion and subsequent metastasis are major characteristics of malignant human renal cell carcinoma (RCC), though the mechanisms remain elusive. Mitochondrial pyruvate carrier (MPC), a key factor that controls pyruvate transportation in mitochondria, is frequently dysregulated in tumor cells and loss of MPC predicts poor prognosis in various types of cancer. However, the clinical relevance and functional significance of MPC in RCC remain to be elucidated. In this study, we investigated the expression of MPC1 and MPC2 in specimens from RCC patients and observed downregulation of MPC1, but not MPC2, in RCC tissues compared with adjacent non-cancerous tissue. Moreover, RCC patients with higher MPC1 expression exhibited longer overall survival rate than those with lower MPC1. Functionally, MPC1 suppressed the invasion of RCC cells in vitro and reduced the growth of RCC cells in vivo, possibly through inhibition of MMP7 and MMP9. Further studies revealed that loss of MPC1 was induced by hypoxia in RCC cells, and notably, MPC1 expression, was negatively correlated with HIF1α expression in RCC cells and patient samples. Taken together, our results identify anti-tumor function of MPC1 in RCC and revealed MPC1 as a novel prognostic biomarker to predict better patient survival.
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Introduction
Renal cell carcinoma (RCC) represents 90–95% of kidney cancer. Although the mortality rate of RCC has been decreasing due to nephrectomy, immunotherapy and targeted therapy, invasion and subsequent metastasis still occur in 25–30% patients and lead to death [1]. Therefore, clarifying the mechanisms that control the invasion of RCC is critical for the development of novel therapeutic strategies.
Accumulating evidence reveals pivotal roles of aberrant mitochondrial metabolism in tumorigenesis [2]. Key molecules associated with mitochondrial metabolism are tightly involved in tumor progression [3]. Mitochondrial pyruvate carrier (MPC), including two members, MPC1 and MPC2, was originally described as gatekeepers responsible for pyruvate transportation and oxidation in mitochondria [4]. Cancer-related functions of MPC have also been identified in several types of cancer. For example, depletion of MPC or blocking its activity by specific inhibitor results in aerobic glycolysis and aggressive growth of tumor cells [2]. Decreased expression of MPC protein is correlated with poor survival of patients with various types of cancer, including colon cancer [5], prostate cancer [6, 7], and esophageal cancer [8]. Overexpression of MPC in colorectal cancer cell lines decreased the tumorigenicity of subcutaneously inoculated tumor cells in mice [5]. Moreover, in colon cancer and esophageal cancer [5, 8], MPC suppresses the Warburg effect, which is one of the most important features in cancer cells. Interestingly, recent work by Ohashi et al. showed that MPC1 is an indicator for favorable prognosis of intrahepatic cholangiocarcinoma and inhibits tumor invasion and metastasis [9]. These results suggest that MPC may function as a tumor suppressor through regulation of metabolism and epithelial–mesenchymal transition (EMT) of tumor cells.
Despite the potential involvement of MPC in cancer invasion and metastasis, the clinical relevance and functional mechanism of MPC in RCC remain to be elucidated. In this study, we investigated the expression of MPC in human RCC and adjacent non-cancerous tissue. Our data suggest that the expression of MPC1, but not MPC2, was downregulated in RCC tissue compared with paired non-cancerous tissue, and the expression levels were negatively associated with tumor size, advanced lymph node metastasis, and overall survival (OS) of patients. Additionally, knockdown of MPC1 increased the migration and invasion of RCC cells. In contrast, overexpression of MPC1 in human RCC cells inhibited their migration, invasion, and tumorigenesis in xenograft models. Mechanistically, we observed that MPC1 expression impaired the mobility of RCC cells potentially through inhibiting hypoxia inducible factor 1α subunit (HIF1α). Thus, our data imply that MPC1 may control the progression of RCC and acts as a novel prognostic indicator for human RCC.
Materials and methods
Patients and tissue specimens
Twelve pairs of tumor and adjacent non-cancerous tissues from RCC patients who underwent surgical resection in the Southwest Hospital, Third Military Medical University (Army Medical University) from January 2014 to December 2016, Chongqing, China with approval from the Institutional Ethics Committee were examined by Western blotting. Two tissue microarrays including 150 RCC tissues without radiotherapy or chemotherapy before surgery were purchased from BioChip (Shanghai, China) (Supplementary Table 1), in which 30 RCC cases included. All patients were followed up after operation. All patients were provided with informed consent. An independent cohort of 164 patient specimens from The Cancer Genome Atlas (TCGA) database was reviewed to validate the prognostic value of MPC1 in RCC.
Immunohistochemical staining
Human tissue sections were deparaffinized and hydrated by a series of xylene and alcohol treatment. The sections were incubated with rabbit polyclonal anti-MPC1 (Abcam, Cambridge, UK), and mouse polyclonal anti-HIF1α (Abcam) antibodies at 4 °C overnight, followed by incubation with avidin–biotin-peroxidase (DAKO). MPC1 and HIF1α were considered positive by cytoplasmic staining and the expression levels were semi-quantitatively analyzed using a composite score system by assessing both the percentage and intensity of stained tumor cells. The percentage of positive cells was calculated in high-power fields (HPF) as follows: [10] sections with <1% positive cells were rated as 0; 1–25% positive cells as 1; 26–50% positive cells as 2; 51–75% positive cells as 3, and 76–100% positive cells as 4. The staining intensity was rated as follows: 1 for weak intensity; 2 for moderate intensity; and 3 for high intensity. Points for staining intensity and the percentage of positive cells were multiplied. Tumor specimens were classified into three groups according to overall scoring: 0–1 as negative expression, 2–4 as weak expression, and 6–12 as high expression. Total scores were expressed as: 0–4 as low and 6–12 as high. All sections were evaluated independently by two pathologists without knowledge of the identity of patients and the clinical outcome.
Reagents
UK5099, a MPC inhibitor, and CoCl2, which elicits a hypoxia condition in cell culture, were from Sigma-Aldrich (St. Louis, MO).
Cell culture
The 786-O RCC cell line was obtained from ATCC and maintained in DMEM supplemented with 10% fetal bovine serum (FBS) (Invitrogen, Carlsbad, CA, USA).
Lentiviral infection
For overexpression of MPC1-Flag in RCC cell line 786-O, full length human MPC1-Flag was generated and inserted into a lentivirus vector. The 786-O cell line (1 × 106/well) was infected with lentivirus containing the constructs. The cells were selected by 4 µg/ml puromycin (Solarbio Science & Technology, Beijing, China). 786-O cells (1 × 106/well) were also infected with siMPC1 or empty control lentivirus vectors (siCtrl) (Genechem, Shanghai, China). The sequences for the siRNAs were: MPC1-RNAi-1 5′-UCCCTCTUTTUCTATTCTTTU-3′, and MPC1-RNAi-2 5′-UUCTTATCAAACACUAUATUA-3′.
Tumor cell invasion and migration assay
Tumor cells in DMEM basic medium (2 × 104/well) were plated in the upper transwells. The lower transwells were filled with DMEM medium containing 10% FBS as cell migration inducer. After 24 h incubation, non-migrating cells were removed from the upper surface of the membranes, and the cells on the lower surface of the membranes were stained with crystal violet (Beyotime, Jiangsu, China). For invasion assay, BD Matrigel™ (BD Biosciences, San Jose, CA, USA) was added into transwell chambers before the cells were seeded. The invading and migrating cells in at least five randomly selected microscopic fields at ×200 magnification were counted under light microscopy. The data was expressed as the mean ± SE of cells per field in triplicate.
Western blotting
Western blotting was performed as previously described [11]. Primary antibodies used were: rabbit polyclonal anti-MPC1 (Cell Signaling, Beverly, MA), rabbit polyclonal anti-MPC2 (Abcam), mouse polyclonal anti-HIF1α (Abcam), rabbit polyclonal anti-MMP7 (Cell Signaling), rabbit polyclonal anti-MMP9 (Cell Signaling), and rabbit polyclonal anti-GAPDH (Cell Signaling) antibodies.
qRT-PCR
Total RNA was extracted from tumor cells with RNAiso reagent (Takara, Dalian, China). Reverse-transcription and quantitative Real-Time PCR were performed by using One Step SYBR Prime Script RT-PCR kit (Takara). Specific primers for amplification were listed in Supplementary Table 2. All experiments were performed in quadruplicate samples with results plotted as the mean ± SD.
Xenografts
All animal experiments were approved by the Institutional Animal Care and Use Committee of the Southwest Hospital, Third Military Medical University (Army Medical University). A total of 1 × 105 MPC1-overexpressing or control cells were suspended in 50 μl PBS and subcutaneously injected into 6-week-old female NOD-SCID mice (Laboratory Animal Center, Southwest Hospital, Third Military Medical University, China). The size of resultant tumors was measured every 7 days for a month using a Vernier caliper and tumor volume was calculated as: shortest diameter2 × longest diameter/2. The animals were sacrificed 30 days after tumor cell implantation and subcutaneous xenograft tumors were analyzed by IHC. Tumor tissue sections deparaffinized and hydrated by a series of xylene and alcohol treatment were incubated with rabbit polyclonal anti-MPC1 (Abcam), rabbit polyclonal anti-MMP7 (Cell signaling), and mouse polyclonal anti-HIF1α (Abcam) antibodies at 4 °C overnight, followed by incubation with avidin–biotin-peroxidase (DAKO).
Statistical analysis
All experiments were performed at least three times with triplicate samples. Statistical analyses were conducted using the SPSS software 18.0. The correlation between MPC1 expression and the clinicopathological features of RCC patients was accessed by the Pearson chi-squared χ2 test. Survival curves were calculated by the Kaplan–Meier method, and comparisons were made by the log-rank test. Paired Student’s t-test for two groups and one-way ANOVA for multiple groups were used. The cutoff value of IHC score was determined by relative risk analysis with the statistical software X-tile [12]. All significant statistical differences were defined as *P < 0.05, **P < 0.01, and ***P < 0.001.
Results
MPC1 is downregulated in RCC tissues
We measured the expression of mitochondrial marker genes (n = 40) in RCC and normal tissues including MPC1, cytochrome C (CYCS), TOMM6, and TOMM7 (Supplementary Figure 1a). MPC1, CYCS, TOMM6, and TOMM7 were examined in 10 pairs of RCC and corresponding adajacent non-cancerous tissue by qPCR. The results showed decreased MPC1 mRNA in RCC tissues compared with adjacent non-cancerous tissues (Supplementary Figure 1b). MPC1 and MPC2 proteins consisting of MPC were examined in 12 pairs of RCC and corresponding adjacent non-cancerous tissue by Western blotting, in which the expression of MPC1 was downregulated in RCC tissues (Fig. 1a, b). However, there was no difference in MPC2 expression in RCC versus adjacent non-cancerous tissue (Fig. 1a, b). Analysis of GEO database (GDS505) showed that the expression of MPC1 mRNA, but not MPC2 mRNA, was decreased in RCC tissues in comparison with normal kidney specimens (Fig. 1c). We also performed IHC using a 150-case RCC cohort (Cohort-150). In 30 paired cases from the Cohort-150, MPC1 expression was lower in tumor tissues than in corresponding adjacent non-cancerous tissue (Fig. 1d and e). In addition, the proportion of MPC1low cells was markedly higher in RCC tissues (60.67%, 91/150) than that in corresponding adjacent non-cancerous tissue (33.33%, 10/30) (Supplementary Table 3). Therefore, our data show downregulation of MCP1 expression in RCC versus corresponding adjacent non-cancerous tissue.
MPC1 expression levels are correlated with the prognosis of RCC patients
We next analyzed MPC1 expression and the clinicopathological features of RCC tissues from Cohort-150. Among these samples, 59 cases were defined as MPC1high and 91 MPC1low. MPC1 expression was negatively correlated with tumor size (T) (P = 0.01) and TNM stages (P = 0.01), but not with tumor location, histological grade, and patient gender (Supplementary Table 4). We also noticed that the expression of MPC1 protein in stage I RCC was significantly lower than that in higher stage RCC (stages II and III) (Fig. 2a, b). These results suggested that decreased expression of MPC1 in RCC was associated with tumor progression. Univariate and multivariate analyses further indicated that low MPC1 expression was an independent prognostic indicator for poor OS of RCC patients (Supplementary Table 5). Kaplan–Meier survival analysis using Cohort-150 showed significantly shorter OS for patients with MPC1low RCC than those with MPC1high RCC (P = 0.0016) (Fig. 2c). Moreover, survival analysis of 164 RCC patients from the TCGA-database consistently indicated that low of MPC1 expression was associated with poorer OS (P = 0.0128) (Fig. 2d). Thus, lower expression of MPC1 acts as an indicator of unfavorable prognosis for RCC patients.
Loss of MPC1 promotes the migration and invasion of RCC cells in vitro
Based on the function of MPC1 in the migration and invasion capacity of cholangiocarcinoma cells [9], we tested the effect of MPC1 on the mobility of RCC cells. We established MPC1-depletion and MPC1-overexpression subpopulations in 786-O cells (Supplementary Figure 2). Transwell assay with or without Matrigel showed that loss of MPC1 increased the migration and invasion capacity of RCC cells (Fig. 3a). Treatment with different concentrations of the MPC inhibitor [13], UK5099, enhanced RCC migration and invasion compared with vehicle-treated cells (Fig. 3b). In contrast, overexpression of MPC1 significantly impaired the migration and invasion of RCC cells in vitro (Fig. 3c). These results suggested that loss of MPC1 was associated with increased mobility and invasiveness of RCC cells.
Matrix metalloprotein, but not EMT, is affected by MPC1 in RCC cells
To define the downstream targets of MPC1 linked to RCC cell migration and invasion, we examined two EMT markers, vimentin and E-cadherin. Neither of the two genes was changed in their levels after MPC1 depletion, MPC inhibitor treatment, or MPC1 overexpression (Supplementary Figure 3a-c). Since matrix metalloproteinases (MMPs) play pivotal roles in cancer cell migration and invasion [14], we then evaluated the expression of MMPs. The mRNA and protein levels of MMP7 and MMP9 were significantly upregulated in 786-O cells transfected with siMPC1 versus siCtrl constructs (Fig. 4a, b). Treatment of RCC cells with the MPC inhibitor, UK5099 also increased the expression of MMP7 and MMP9 (Fig. 4c, d). However, RCC cells overexpressing MPC1 showed reduction in the expression of MMP7 and MMP9 (Fig. 4e, f). Thus, loss of MPC1 enhances the mobility and invasiveness of RCC cells through upregulation of MMPs.
MPC1 is involved in the pathway of hypoxia/HIF1α axis in RCC cells
Hypoxia frequently occurs in RCC [15] that leads to increased cell migration and invasion [16, 17]. In addition, proteins in mitochondria are highly sensitive to regulation by hypoxia [18, 19]. We therefore speculated that MPC1 might be regulated by hypoxia. CoCl2 treatment or culture under 1% O2 to mimic hypoxia [20], reduced the expression of MPC1 in RCC (Fig. 5a and Supplementary Figure 4a). Since HIF1α is a crucial regulator of hypoxia [21] and is linked to the progression of RCC [22], we investigated the relationship between MPC1 and HIF1α. We observed that suppression of MPC1 expression increased HIF1α expression in RCC (Fig. 5b and Supplementary Figure 4b). In contrast, overexpression of MPC1 decreased HIF1α (Fig. 5c). Moreover, in specimens from Cohort-150, we found that the expression levels of HIF1α in MPC1low RCC cells were higher than that in MPC1high RCC cells (Fig. 5d, e), a finding also confirmed using TCGA database (Supplementary Figure 4c). These results suggest the possibility of MPC1 as an upstream regulator of HIF1α in RCC cells.
Overexpression of MPC1 reduces the growth of RCC xenograft tumor
To examine the effect of MPC1 on the malignant behavior of RCC cells in vivo, RCC cells (786-O) with overexpressing or knockdown MPC1 were subcutaneously implanted into immunocompromised mice (Fig. 6a, b). The average weight and volume of tumors formed by RCC cells with MPC1 knockdown grew more rapidly than those formed by RCC cells overexpressing MPC1 (Fig. 6c, d). IHC staining of xenograft tumors showed that the expression of MMP7 and HIF1α was decreased in tumors derived from MPC1 overexpressing RCC cells as compared with those derived from Mock-transfected cells (Fig. 6e). Therefore, xenograft data demonstrate that higher level of MPC1 expression is associated with decreased rate of the growth of RCC and the expression of MMP7 and HIF1α in tumors.
Discussion
RCC contains metabolic alterations characterized by increased glycolysis rate and lactate production under normoxia condition known as Warburg effect [23], which is correlated with pyruvate metabolism and attenuation of pyruvate transportion [24]. Therefore, pyruvate-transporting-related proteins may play critical roles during tumorigenesis and tumor progression. In this study, we report that MPC1 was significantly downregulated in RCC tissues, with more advanced stages and poorer OS of patients. In vitro, the migration and invasion of RCC cells were enhanced when MPC1 was depleted with siRNA or suppressed by MPC inhibitor. Thus, MPC1 acts as a tumor suppressor in RCC.
It has been reported that MPC1 expression is reduced in various types of cancer [25,26,27]. Deficiency of MPC1 contributes to the dysregulation of mitochondria-related metabolism that leads to oncogenic switch of metabolic profiles [5, 27]. In cancer cells, MPC1 deficiency or mutation-mediated inactivation promote aerobic glycolysis and stemness [28]. Although both MPC1 and MPC2 are simultaneously downregulated in some types of cancer [5,6,7], we observed that only MPC1 was aberrantly regulated in RCC. MPC1 and MPC2 form a multimeric complex to control pyruvate transportation [4, 29], and both are required for complex stability [5, 30]. Therefore, either MPC1 or MPC2 aberrant regulation may result in functional loss to promote tumorigenesis. It has been found that suppression of MPC1 is sufficient to interfere with mitochondrial pyruvate uptake and oxidation critically involved in Warburg effect in cancer cells [5].
Low expression of MPC1 in cancer cells leads to increased cell glycolysis which is responsible for enhanced invasive phenotype [6, 31]. Hypoxia is also tightly associated with glycolysis, and plays pivotal roles in promoting tumor progression, including RCC [15, 32]. We observed that hypoxia in RCC cells resulted in MPC1 downregulation and MPC1 depletion increased HIF1α expression. Moreover, MMPs, but not EMT, was upregulated by MPC1 deficiency, which may be responsible for increased invasiveness of RCC cells.
In conclusion, loss of MPC1 expression in RCC predicts poor survival of patients. MPC1 reduces the mobility and invasiveness of RCC cells by inhibiting HIF1α and MMPs expression. Therefore, MPC1 plays a tumor-suppressor function in RCC and acts as a prognostic indicator.
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Acknowledgements
This project was supported by grants from the National Natural Science Foundation of China (Nos. 81372684 and 81230062).
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Tang, XP., Chen, Q., Li, Y. et al. Mitochondrial pyruvate carrier 1 functions as a tumor suppressor and predicts the prognosis of human renal cell carcinoma. Lab Invest 99, 191–199 (2019). https://doi.org/10.1038/s41374-018-0138-0
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DOI: https://doi.org/10.1038/s41374-018-0138-0
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