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The effect of the placental DROSHA rs10719 and rs6877842 polymorphisms on PE susceptibility and mRNA expression

Journal of Human Hypertension volume 33, pages 552–558 (2019)Cite this article

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Abstract

Evidence showed that microRNA biosynthesis plays the main role in pathogenesis of several diseases including Preeclampsia (PE). Therefore, microRNA processing enzymes may involve in PE predisposition. The aim of the present study was to evaluate the relation between DROSHA rs10719 and rs6877842 polymorphisms and mRNA expression in the placenta of PE women and controls. This study recruited 110 PE women and 115 age matched normotensive pregnant women for genotyping of DROSHA polymorphisms and analyzing of mRNA expression. There was no association between alleles and genotypes of placental DROSHA rs10719 and rs6877842 polymorphisms and PE susceptibility. However, placental DROSHA rs10719 was associated with increased PE risk in the recessive model. The combination of CC/GG genotypes of DROSHA rs10719 and rs6877842 polymorphisms was associated with higher risk of PE. The frequency of C-G haplotype was higher in PE women, but the difference was not significant. The DROSHA mRNA expression was downregulated in the placenta of PE women. There was no relation between DROSHA mRNA expression and rs6877842 polymorphism, however, it was decreased in the placenta of women with rs10719CC genotype. The placental DROSHA rs10719 but not rs6877842 polymorphism could be a risk factor for PE susceptibility only in the recessive model. The combination of CC/GG genotypes could be risk factors for PE susceptibility. The DROSHA expression downregulated in the preeclamptic placentas and those carrying rs10719CC genotype.

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Acknowledgements

This article was extracted from an MS thesis (IR.ZAUMS.REC.1395.198) at Zahedan University of Medical Sciences. The authors would like to thank the Deputy of Research Affairs at the University for funding this project.

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Authors and Affiliations

  1. Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

    Mahnaz Rezaei, Abbas Mohammadpour-Gharehbagh & Saeedeh Salimi

  2. Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran

    Abbas Mohammadpour-Gharehbagh & Saeedeh Salimi

  3. Department of Medical Genetics, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

    Mehrnaz Narooei-nejad

  4. Department of Obstetrics and Gynecology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

    Batool Teimoori

  5. Department of Obstetrics and Gynecology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

    Mojgan Mokhtari

  6. Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran

    Mehrnaz Mehrabani

  7. Department of Obstetrics and Gynecology, School of Medicine, Shahid Beheshty University of Medical Sciences, Tehran, Iran

    Minoo Yaghmaei

  8. School of Medicine, Iran University of Medical Sciences, Tehran, Iran

    Darya Najafi

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  1. Mahnaz Rezaei
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Correspondence to Saeedeh Salimi.

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Rezaei, M., Mohammadpour-Gharehbagh, A., Narooei-nejad, M. et al. The effect of the placental DROSHA rs10719 and rs6877842 polymorphisms on PE susceptibility and mRNA expression. J Hum Hypertens 33, 552–558 (2019). https://doi.org/10.1038/s41371-018-0156-9

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