Abstract
Introduction: Iron (Fe), copper (Cu) y zinc (Zn), are trace essential microminerals for life. Its deficiencies or excess are deleterious for human. The uptake of these metals is in the duodenum and small intestine. For the uptake, these metals use and share metals transporters localized in the apical membrane of the intestinal epithelial cells.
Objective: To determine the effect of different molar ratios of Fe, Cu y Zn over their uptake and its interactions in intestinal epithelial cells (Caco-2).
Methods: Caco-2 cells were grown with different molar ratios of Fe, Cu y Zn: a) directly in the culture media or b) in an in vitro digest of milk supplemented with the metals and we measured their uptake and studied its intracellular bioavailability with ferritin (Fn), metalothionein (MT) y CCS.
Results: Fe uptake was inhibited by Cu y Zn. Cu uptake was inhibited only by Fe. A 1:1:1 molar ratio of Fe:Cu:Zn inhibited (45%) the uptake of Fe or Cu. Fn and CCS expression increased synergically when two metals were in the culture media. MT expression increased only when cells were incubated with Cu or Cu/Fe.
Conclusion: These results suggest that increased molar ratios may produce an inhibition in the uptake of one or two metals. Supported by Universidad de Chile, DI TNAC 07/03
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Arredondo, M., Le Blanc, S., Silva, C. et al. Negative Interacctions Between Fe, Cu y Zn: TL032. Pediatr Res 60, 637 (2006). https://doi.org/10.1203/00006450-200611000-00034
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DOI: https://doi.org/10.1203/00006450-200611000-00034