Abstract
Aim: To explore the Mechanism of inhibited peripheral blood lymphocyte apoptosis in children with Kawasaki disease. Methods: Calculating percentage of apoptotic cells and assaying DNA fragmentation in peripheral blood lymphocyte(PBL) in vitro from 30 kawasaki disease(KD) patients and 20 age-matched healthy children, measuring in vitro production of IL-6 by ELISA, expression of PBL p53 gene expression using dot-blot and flow cytometry (FCM). Results: Apoptotic cell percentage and DNA fragmentation were markedly decreased (P<0.001) and delayed,compared with those in normal controls, which could reach the states of the control group only being cultured for 72h, remarkebaly increased production of IL-6 in cultured supernatant of PBL induced by phytohemagglutinin (PHA) from KD patients was found, and the expression of PBL p53 gene expression was decreased in KD patients. Adding anti-IL-6 monoantibody(mAb) to the cultures or using intravenous immunoglobulin (IVIG) in vivo led to significantly decreased production of IL-6(P<0.001), and reversed decreased apoptotic cell percentage and delayed DNA fragmentation, while the expression of PBL p53 gene increased. Conclusion: The data suggested that PBL apoptosis is delayed in KD patients. It might be related to over-production of IL-6 inhibiting expression of p53 gene.
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Yi, QJ., Li, CR. & Yang, XQ. Study on the Mechanism of Inhibited Peripheral Blood Lymphocyte Apoptosis in Children with Acute Kawasaki Disease. Pediatr Res 53, 170 (2003). https://doi.org/10.1203/00006450-200301000-00101
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DOI: https://doi.org/10.1203/00006450-200301000-00101