Abstract 33
Background: BPD or chronic lung disease is a prolonged respiratory failure and cytokines have been implicated in the development of CLD. Steroids have produced improvement in neonates with BPD.
Aim: To evaluate the downregulation of cytokines by steroids and rIL-10 in vitro.
Methods: Human macrophages differentiated from a monocytic cell line (THP-1), a rat alveolar macrophage cell line (ATCC 8383) and human lung macrophage from bronchoalveolar lavage (BAL) were stimulated with Ureaplasma urealyticum antigen or LPS in combination with different doses of dexamethasone or budesonide or rIL-10. TNFα and IL-6 from the supernatant were measured with ELISA or bioassay.
Results: Either dexamethasone, budesonide or rIL-10 could significantly inhibit the IL-6 and TNFα production in THP-1 cell line; They have similar effects to TNFα production in the BAL macrophage; In the rat macrophage cell line both dexamethasone and budesonide could significantly inhibit the IL-6 and TNFα production but not rIL-10.
Conclusion: Steroids and rIL-10 could be important therapeutic factors by downregulating the proinflammatory cytokines production.
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Li, Y., Brauner, A., Jonsson, B. et al. Inhibition of macrophage proinflammatory cytokines by steroids and rIL-10. Pediatr Res 45, 892 (1999). https://doi.org/10.1203/00006450-199906000-00051
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DOI: https://doi.org/10.1203/00006450-199906000-00051
