Abstract 8

Magnesium has been proposed as a potential therapeutic strategy of hypoxic-ischemic insult for their ability to block the entry of Ca2+ through NMDA receptor. Objective: The aim of this work was to examined the neuroprotector effect of magnesium sulfate (MgSO4) in a experimental model of hypoxicischemic insult when is administrated as a rescue strategy, 15 minutes after the insult. Methods: Piglets (5 days) were assigned to (S) Sham (n=8), (SM) Sham+MgSO4 (n=8), (HI) hypoxic-ischemic (n=8), or (HIM) hypoxic-ischemic+MgSO4 (n=8) group. In the groups HI and HIM temporary occlusion of the carotid arteries during 30 min (FiO2 8%), and hemorragic hypotension (15 min) after 15 min of hypoxic insult was made. A loading dosis of 300 mg/kg of MgSO4 followed by 150 mg/kg/h i.v. were administrated during 12h to SM and HIM groups. NSE levels were measured by EIA. Cerebral histologyc examination was performed; neuronal necrosis was assessed in differents regions of the brain. Results: Significant increase in NSE levels in the sacrifice time were found in the HI and HIM groups (p<0.05). Severe brain damage was found in groups HI and HIM in any of the sections examined, mainly in cortex, basal ganglia and hippocampus. Conclusions: We don't found neuroprotector effect when MgSO4 was administrated 15 minutes after the hypoxic-ischemic insult in this experimental model. Supported by grant from the ARBORA S.A. Figure

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