We have previously defined patterns of regulation of intestinal apolipoprotein (apo) expression by acute (24 hr) lipid absorption in the newborn piglet. Purpose: To determine the effect of chronic (1 week) feeding of dietary triglyceride (TG) of varying fatty acid composition on small intestinal and hepatic apo expression in newborn swine. Methods: Two-day-old female swine were fed one of three diets by gavage with the following lipid composition: MCT (medium-chain TG, MCT oil), ICST (intermediate-chain saturated TG, coconut oil), and LCPUT (long-chain polyunsaturated TG, safflower oil) at 180 kcal/kg/day with 51% of calories from fat. After 1 week, serum lipids and apo levels were measured, and jejunal and ileal apo B and A-I mass and synthesis were measured. Liver was processed for determination of apo B and A-I mass and mRNA abundance by slot blot hybridization. Results: Serum lipid and apo levels [mg/dL, mean(SEM)] are shown in the table below (*different from the other groups at p<0.05, N=6 for each group). There were no differences among the three groups for intestinal apo mass (ng/μg protein) or synthesis (% total protein synthesis), except higher apo B mass in MCT ileum. In liver, apo A-I mass was highest in the ICST group (0.48±0.04) compared to the MCT(0.30±0.03) and LCPUT (0.39±0.03) groups (p<0.01). Liver apo A-I mRNA levels paralleled mass levels (p<0.05). Conclusion: Chronic feeding of ICST in newborn swine increases serum total CH, TG, HDL-CH, and apo A-I levels, compared to feeding MCT or LCPUT. Increased hepatic apo A-I expression may contribute to the higher serum apo A-I levels in the ICST animals.