Abstract
The FIA methods show better sensitivity and specificity than RIA. Normal reference values have not been determined for children. We measured serum LH and FSH levels under basal conditions and after GnRH stimulation (100ug iv) in 21 normal children (11 boys, ages 4-11 years, and 10 girls, ages 4-10 years)using RIA and FIA methods. All children had Tanner I pubertal development and prepubertal serum levels of testosterone and estradiol. RIA measurements were performed by double antibody kits purchased from Pharmacia. Sensitivity was 0.95 IU/L for LH and 2.5IU/L for FSH. FIA determinations were obtained with Delfia LH Specific and FSH kits kindly provided by Pharmacia. The intra and interassay coefficients of variation were below 5 and 12% respectively.
FIA measurements basal and after GnRH stimulation are lower than RIA. Thus normal reference values obtained with RIA methods cannot be used in the interpretation of FIA measurements. The results may be used as FIA reference velues for children.
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Mendonca, B., Borges, M., Batista, M. et al. 27 COMPARISON OF IMMUNOFLUORIMETRIC (FIA) AND RADIOIMUNOASSAY (RIA) METHODS FOR HE HEASUREMENT OF BASAL AND GnRH STIMULATED LEVELS OF GONADOTROPINS IN NORMAL CHILDREN. Pediatr Res 36, 676 (1994). https://doi.org/10.1203/00006450-199411000-00051
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DOI: https://doi.org/10.1203/00006450-199411000-00051