Abstract
From a population-based study cohort of 1812 elementary school children we selected 129 nine year old unrelated children to investigate the clinical relevance of HLA-cIass II molecules in the regulation of immune response to the dust mite allergen Der p I. Individuals were selected on the basis of skin prick test results which were validated by measurement of specific IgE (sIgE). Three different groups were defined according to sIgE determinations: Group I (n=20) included controls without detectable sIgE; in group II (n=22) were patients with sIgE to antigens other than Der p I; group III patients (n = 85) had sIgE to Der p I and other allergens. In total, 43 different HLA class II alleles were determined by sequence specific oligonucleotide typing with PCR amplified patient DNA. Gene frequencies were determined for every allele in the three study groups and no statistically significant difference could be demonstrated between children sensitized to Der p I and controls. In addition, the association of HLA class II haplotypes with clinical phenotypes was investigated. A positive association was found between DRB *0100/*0300/*1100 and/or DPB *0201/*0401 in patients suffering from asthma, hay fever or atopy (p≤0.01). Detection of a rising number of the above mentioned alleles was associated with an increasing risk of presenting with a clinical history of allergic disease. Similarily, there is evidence for a negative association between the presence of DQB *0303/*0503 and/or DRB *0200/*0700 and a clinical history of eczema, hayfever and atopy (p≤0.01). Thus, the findings of our epidemiological study do not confirm the clinical relevance of HLA-class II molecules for IgE responses to dust mite allergen. However, certain HLA haplotypes were clearly associated with a significantly increased risk for the presence of allergic disease.
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Stephan, V., Schmid, V., Wahn, V. et al. 325 DUST MITE ALLERGY AND RESTRICTION BY HLA-CLASS II IMMUNE RESPONSE GENES. Pediatr Res 36, 57 (1994). https://doi.org/10.1203/00006450-199407000-00325
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DOI: https://doi.org/10.1203/00006450-199407000-00325