Abstract
Introduction: In children who survive congenital diaphragmatic hernia (CDH) the incidence of bronchopulmonary dysplasia is more than 30% (Pediatr Pulmonol 1993:15,231). This may be due a failing antioxidant enzyme activity (AOA). Therefore the effect of artificial ventilation on AOA in an experimental model of CDH was studied.
Methods: In neonatal rats CDH was induced by Nitrofen given at day 12 of gestation (J Pediatr Surg 1990;25:850). Pressure-controlled ventilation was performed with 100% oxygen, freqency 40/min, inspiratory peak pressure of 17 cm H2O and PEEP 3 cm H2O. The lungs were examined for activity of glutathione peroxidase (GPX), catalase (CATA) and glutathione reductase (GR) directly after birth and after six hours of artificial ventilation.
Results: AOA activity, all values expressed as IU/mg lung, mean (SE).
At t=0 CATA and GPX are significantly higher in CDH compared to control rats (p<0.001). After six hours the AOA increases significantly (p<0.05) for all enzymes in both groups.
Conclusion: Immediately after birth no deficient AOA was found in CDH. Whether the observed increase in AOA in CDH reflects adaptation to the artificial ventilation remains speculative.(Supported by NAF 91.56).
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Ijsselstijn, H., Van Miert, M., Sluiter, W. et al. 281 ALTERED ANTIOXIDANT ENZYME ACTIVITY IN LUNGS OF NEONATAL RATS WITH CONGENITAL DIAPHTAGMATIC HERNIA DURING ARTIFICAL VENTILATION. Pediatr Res 36, 49 (1994). https://doi.org/10.1203/00006450-199407000-00281
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DOI: https://doi.org/10.1203/00006450-199407000-00281