Abstract
ABSTRACT: The higher susceptibility to serious bacterial infections of patients, particularly children, infected with the human immunodeficiency virus (HIV) may be due in part to defective function of their phagocytic cells. We examined the ability of polymorphonuclear cells and monocytes of HIV-infected children and adults to generate superoxide anion (SO) and hydrogen peroxide (HP) and compared it with that of cells from normal children and adults. SO was measured by reduction of cytochrome c and HP by horseradish peroxidase-dependent oxidation of phenol red. The cells were incubated in 96-well plates at 37°C for 2 h before the assay and the nonadherent cells then removed. Readings for SO were taken at 10, 30, 60, and 120 min after stimulation with phorbol myristate acetate; HP production was assayed after 90 min. The SO and HP production by polymorphonuclear cells and monocytes from both HIV-infected children and adults was consistently found to be markedly lower than that of cells from age-matched controls. The magnitude of the difference in response between patients and control cells also increased with increasing incubation time. Thus, phagocytic cells from HIV-infected children and adults are defective in their ability to generate reactive oxygen intermediates, and this defect may make them more vulnerable to bacterial and fungal infections.
Similar content being viewed by others
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chen, T., Roberts, R., Wu, K. et al. Decreased Superoxide Anion and Hydrogen Peroxide Production by Neutrophils and Monocytes in Human Immunodeficiency Virus-Infected Children and Adults. Pediatr Res 34, 544–550 (1993). https://doi.org/10.1203/00006450-199310000-00032
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1203/00006450-199310000-00032
This article is cited by
-
NADPH containing superoxide-producing thermostable complex from raspberry, apricot, grape, and grape seeds: isolation, purification, and properties
Plant Methods (2023)
-
Effect of Antiretroviral Therapy on Neutrophil Oxidative Burst in Children
Indian Journal of Pediatrics (2023)
-
Superoxide-producing thermostable associate from the small intestines of control and alloxan-induced diabetic rats: quantitative and qualitative changes
BMC Endocrine Disorders (2022)
-
The role of polymorphonuclear neutrophils during HIV-1 infection
Archives of Virology (2018)