Abstract
Serum gonadotropin responses after GnRH stimulation and secretion of LH and FSH in nocturnal urine samples were measured by ultrasensitive time-resolved immnunofluorometric assay, for 95 subjects (62 boys, 33 girls; age 2-14 yrs) with non-endocrine short stature or obesity. The subjects were classified according to sex and pubertal stage. The prepubertal children were divided into two groups, according to chronological age (below or above 10 yrs). The basal and peak serum LH levels after GnRH stimulation and urinary LH showed a gradual increase throughout the pubertal development, while changes in FSH were not as remarkable as for LH. The peak LH/peak FSH ratio after GnRH stimulation and urinary LH/FSH ratio reliably discriminated between prepubertal and early pubertal groups. The reference ranges (mean-SD-mean+SD, after logarithmic transformation) of serum peak LH/peak FSH ratio in prepubertal group and early pubertal group were 0.28-0.55, 1.4-3.4 in boys and 0.09-0.25, 0.74-1.4 in girls, respectively, while urinary LH/FSH ratio in each group ranged 0.03-0.13, 0.37-1.1 in boys and 0.02-0.07, 0.14-0.36 in girls, respectively.
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Ito, J., Tanaka, T., Horikawa, R. et al. CHANGES IN SERUM AND URINARY LH/FSH RATIO DURING PUBERTY. Pediatr Res 33 (Suppl 5), S86 (1993). https://doi.org/10.1203/00006450-199305001-00498
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DOI: https://doi.org/10.1203/00006450-199305001-00498