Abstract
The role of the kidney in the metabolism of prolactin (PRL) in humans has not yet been established, though previous rat model studies showed a 67 % contribution of kidney to PRL metabolic clearance rate. To investigate her urinary PRL (u-PRL) represents an index of renal proximal tubular function, a time-resolved fluoro-immuuno assay was developed. After dialyzation/concentration of urine samples, recovery rate of added PRL was (M ± SD) 97.0 ± 14.4 %, while intra- and inter-assay variations (CV %) were. 4 .2 ± 3.4 and 6.1 ± 4.3 %. Lower limit of assay was 0.25 ng/ml. Immuno cross-reactivity to GH was 0.0032 % at a maximal concentration of 300 ng/ml. Urine samples of 30 normo-prolactinemic subjects (M:9, F:21, age 14.6 ± 5.4 y) with renal disease and associated proteinuria were tested. u-PRL concentration range 4.2 to 12, 824 pg/ml (2.2-17, 330 ng/g CR). u-PRL concentrations were significantly correlated with urinary beta−2 microglobulin (r =0.86, p<0.001), and with serum BJN (r=0.46, p<0.001). Thus, u-PRL is proved to be an additional index of renal proximal tubular function.
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Felice, C., Tanaka, T., Itoh, J. et al. URINARY PROLACTIN: A NEW MARKER FOR THE RENAL PROXIMAL TUBULAR FUNCTION IN CHILDHOOD. Pediatr Res 33 (Suppl 5), S34 (1993). https://doi.org/10.1203/00006450-199305001-00187
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DOI: https://doi.org/10.1203/00006450-199305001-00187