Abstract
Restriction fragment length polymorphisms(RFLP) of P450c11 gene were studied among the Japanese Population using P450c11 cDNA clone as a probe. Genomic DNAs from 60 unrelated Japanese individuals were analyzed by Southern blot hybridization. Two RFLPs were detected in Msp I digests of the DNAs. One(A) was characterized by polymorphic bands at 3.4 and 2.5 kilobase-pairs(kbp) and the other(B) was by polymorphic bands at 1.7 and 1.2 kbp. In A RFLP, 17 of the 60 persons (28%) were homozygous for the 3.4 kbp band, 7(12%) for the 2.5 kbp band and 36(60%) were heterozygous for both bands. In B RFLP, 50 of the 60 persons(83%) were homozygous for the 1.2 kbp band, 10(17%) were heterozygous for the 1.7 and 1.2 kbp bands but homozygotes of the 1.7 kbp band were not detected. And the third RFLP characterized by polymorphic bands at 5.8 and 4.0 kbp was detected in Pvu II digests. Of the 60 persons, 44(73%) were homozygous for the 5.8 kbp band, 16(27%) were heterozygous for both bands but no homozygotes of the 4.0 kbp band were detected. These polymorphisms were different from previously reported Caucasian RFLP.
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Nakagawa, Y., Takeuchi, H., Kubota, A. et al. RACIAL DIFFERENCE OF RESTRICTION FRAGMENT LENGTH POLYMORPHISMS OF P450c11 GENE. Pediatr Res 33 (Suppl 5), S23 (1993). https://doi.org/10.1203/00006450-199305001-00118
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DOI: https://doi.org/10.1203/00006450-199305001-00118