Abstract
The aim of this work was to study autologous immunomodulation (vaocination mechanisms) on autoimmune pancreatic aggression. Splenocytes frcm multiple-low-dose streptozotocin diabetic mice were transferred to normal syngeneic mice. Recipient animals developed abnormal glucose tolerance and diminished 1st. and 2nd. phases of insulin secretion. When splenocytes from diabetic donors were incubated with Mitomycin C prior to transfer, cells remained viable but they loose their pancreatic aggression ability. Splenocytes from diabetic donors were incubated with Mitomycin C and then injected into syngeneic normal mice. 15 days after, mice were injected with an equal dose of splenocytes from diabetic donors. 15 days after the last injection, recipients showed glucose tolerance and insulin secretion profiles similar to control groups in 50% of the injected animals. This protective effect was specifically induced by splenocytes from diabetic mice incubated with Mitomicyn C prior to transfer (controls: 1065±17 vs. diabetics:1078±20 uU insulin/4 min/100 mg w.t., n=6). This effect was not observed in athymic recipients, suggesting that an immune response should be mounted in recipient animals. These results show that Mitomycin C-incubated splenocytes from diabetic donors specifically induced a protective effect against immune aggression and represented an experimental model to study autologue immunomodulation mechanism in autoimmune diabetes.
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Arata, M., Quintanas, C. & Basabe, J. 33 AUTOLOGOUS IMMUNOMODULATION OF PANCREATIC AGGRESSION IN AN EXPERIMENTAL MODEL OF AUTOIMMUNE DIABETES. Pediatr Res 32, 254 (1992). https://doi.org/10.1203/00006450-199208000-00056
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DOI: https://doi.org/10.1203/00006450-199208000-00056