Abstract
IGF-II and the IGF-II/M6P receptor are thought to play an important role in fetal growth and development. We have studied the expression of the IGF-II/M6P receptor in fetal bovine tissues from 5 weeks through 36 weeks gestation. Tissues from bovine fetuses were extracted in buffer containing 2% Triton-X-100 and 2% SDS. Aliquots of the protein extracts were analysed by SDS-PAGE and the protein bands were transfered onto nitrocellulose. Immunoblotting was performed using anti-IGF-II/M6P receptor antiserum. In a subset of experiments, ligandblotting was carried out using radiolabeled IGF-II and subsequent autoradiography. IGF-II/M6P receptors were expressed in all tissues examined, with the highest amount of receptor being present in fetal lung and liver. Low amounts of receptors were measured in fetal brain. The amount of receptor was developmental regulated throughout fetal life. Developmental regulation of receptor expression varied among the different tissues. In conclusion, the IGF-II/M6P receptor is developmentally regulated during bovine fetal life. We hypothesize that this receptor exerts important biologic effects during fetal development, (supported by DFG grant, Ki 365 1.1).
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Pluender, M., Sauerwein, H., Funk, B. et al. 155 EXPRESSION OF THE INSULIN-LIKE GROWTH FACTOR-II/MANNOSE-6-PHOSPHATE RECEPTOR IN FETAL BOVINE TISSUES. Pediatr Res 30, 654 (1991). https://doi.org/10.1203/00006450-199112000-00185
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DOI: https://doi.org/10.1203/00006450-199112000-00185