Abstract
Introduction: Transport of urea through the blood-brain barrier (BBB) and superoxide anion radical production on Che brain's surface are both increased during post-ischemic reperfusion.
Study objective: To see if the alteration of the BBB penetration to urea could be linked to superoxide anion generation, 20 minutes of total cerebral ischemia followed by 2 hours reperfusion was produced by increasing intracranial pressure with a hollow parietal bolt.
Interventions: Newborn pigs were pretreated with systemic polyethylene glycol-superoxide dismutase (PEG-SOD), 1000 U/kg, and polyethylene glycol-catalase (PEG-CAT), 10,000 U/kg. 14C-Urea transfer into the brain was measured in pretreated (n=5) and in control animals (n=5).
Main results: Transfer of 14C-Urea into the brain is not significantly altered by pretreatment with PEG-SOD and PEG-CAT; Kin transfer constant = 37.0 ± 1.6 in treated group vs 34.0 ± 2.4 cm3·g−1·s−1·106 in control group (mean ± SEM).
Conclusion: The results of the present study suggest that superoxide anion generation does not directly contribute to the alteration of urea transfer through the BBB during ischemia-reperfusion in the newborn pig.
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Thélin, O., Mirro, R., Armstead, W. et al. 75 EFFECT OF POLYETHYLENE GLYCOL-SUPEROXIDE DISMUTASE AND CATALASE ON BLOOD-BRAIN BARRIER PERMEABILITY AFTER ISCHEMIA-REPERFUSION IN THE NEWBORN PIG. Pediatr Res 28, 289 (1990). https://doi.org/10.1203/00006450-199009000-00099
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DOI: https://doi.org/10.1203/00006450-199009000-00099