Abstract
Parenterally administered epidermal growth factor (EGF) produces trophic effects on various organs of suckling mammals. Present studies investigated distribution of intravenously administered rat EGF (rECF) in liver and GI tract of 12-day-old suckling rats anesthetized with fentanyl (6 μl/rat). The bile duct was cannulated, and 125I-rEGF (2-4 ng/rat) was administered via femoral vein. After 5, 30, 60 and 120 min, animals were killed and extracted radioactivity (RA) from tissue was characterized by immunoaffinity chromatography. The highest levels of RA were found in liver (46.1±3.0% of total); and 98% was immunoreactive (IR) at 5 min. Both total and IR-RA in liver decreased with time. Large amounts of administered RA were detected in bile at 60 min (2.2%) and 120 min (5.1%); more than 99% of the RA was IR. Concentrations of RA in bile were 15 X greater than that in blood. RA in wall and luminal flushings of stomach and small intestine increased with time. More than 95% of the RA extracted from wall and luminal flushings of small intestine was found to be IR. Cannulation of the bile duct caused a significant reduction in the levels of RA in luminal flushing of the small intestine; however, the percentage of IR was unaffected. IR in the wall and flushings of stomach was more than 60% at 30 min, decreasing to 7-9% at 60 and 120 min. These results suggest that IR EGF is secreted into the lumen via bile, as well as across the wall of the GI tract in suckling rats.
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Koldovský, O., Kong, W., Davis, T. et al. INTRAVENOUSLY ADMINISTERED 125I-EGF IS SECRETED INTO BILE AND LUMEN OF SUCKLING RAT STOMACH AND INTESTINE. Pediatr Res 27, 539 (1990). https://doi.org/10.1203/00006450-199005000-00082
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DOI: https://doi.org/10.1203/00006450-199005000-00082