Abstract
Entire small intestine has not been used for transport studies in animal models of rotavirus (RV) diarrhoea. We have studied water and electrolyte transport in a neonatal rat model of RV diarrhoea using in situ steady state perfusion of the whole small intestine. Perfusion with an isotonic plasma electrolye solution without glucose at 48 hours post oral challenge with rate RV showed net water secretion (-21.5 ±7.3 ul; Cont: +67.9 ± 8.5 ul/min/g dry weight of gut). To determine whether net water secretion can be reversed by solutions containing glucose (G) or glucose polymer (GP), the rats were perfused with six different solutions. Two were hypertonic: A) Na 35, C 200 mM/L; B) Na 9D, G 111; and four were hypotonic: C) Na 60, G 90; D) Na 60, G 111; E) Na 60, GP 9; and F) Na 60, CP 18 mM/l. Hypertonic solution A, containing low sodium and high glucose, caused further secretion (-38.2 ± 8.0 ul/min/g). The other hypertonic and all hypotonic solutions reversed secretiont o absorption, although net water absorption was always significantly greater from hypotonic solutions, being maximal from the solution containing 18 mM GP. Solutions containing 60 mM sodium showed net sodium secretion, 90 mM induced absorption. This model shows that RV causes a secretary state in the small intestine, which can be reversed by glucose electrolyte solutions. Maximal water absorption occurs from a hypotonic solution containing GP. High concentration of glucose leading to hyperosmolality causes secretion in rotavirus diarrhoea.
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Salim, A., Walker-Smith, J. & Farthing, M. INTESTINAL WATER AND SOLUTE TRANSPORT IN A NEONATAL RAT MODEL OF ROTAVIRUS DIARRHOEA. Pediatr Res 26, 280 (1989). https://doi.org/10.1203/00006450-198909000-00100
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DOI: https://doi.org/10.1203/00006450-198909000-00100