Abstract
We have previously shown that chloecystokinin (CCK) accerlerates the movement of immunoglobulins across the intestinal mucosa. This study investigates some mechanisms of this phenomenon, Hooded-Lister rats (160-180 g) were sensitized with 250ng Ovalbumin with Freund's complete adjuvant. On the 14th day a booster injection was given. On the 21st day the rats were anaesthetised and O.SnCi radiodinated human serum albumin was given i.v. A segment of intestine 10 cm long was isolated 10 cm. distal to the pylorus and perfused with 0.9% NaCl at a rate of 0.5 ml/min. The calcium channel blocker, Verapamil (50 g/ml) was injected i.v. 0.025 ml (1 ng) every 30 seconds for 20 mins. Ten mins. after starting Verapamil, 20 ng of CCK-octapeptide (CCK-OP) was given i.v. A control group received CCK-OP only. While in the control group there was a 20% rise of IgA and a 15% rise of ICG (P < 0.01) above baseline. Verapamil prevented this change. A second group received 0.2 mg of the chloride channel blocker, Furosemide (F) i.v. and 0.5 mg in the perfusate. F. also prevented the CCK-induced rise of antibodies. The difference between the treated and the control groups remained significant for about 12.5 minutes. The loss of albumin at 2.5 minutes was 35% above the baseline after CCK and only 13% above if F. was added before the CCK (P < 0.001). It is concluded that 1) CCK provokes an increased shift of albumin, IgA and IgG into the lumen of the intestine. (2) CCK causes a rise of cystolic calcium. (3) CCK-induced translocation of albumin, IgG and IgA i s prevented by F.
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Freier, S., Ran, M. & Faber, J. THE EFFECT OF CALCIUM AND CHLORIDE CHANNEL BLOCKERS ON THE MOVEMENT OF ANTIBODIES AND ALBUMIN IN THE RAT INTESTINE. Pediatr Res 26, 268 (1989). https://doi.org/10.1203/00006450-198909000-00027
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DOI: https://doi.org/10.1203/00006450-198909000-00027