Abstract
After overnight fasting, a water overload test(470ml/m2 for 2.5 hours)was done in 11 normal children(N) (7males, 4females) and 22 insulin-dependent diabetic children (D) (9males, 13females) ranging from 1 to 14 years of diabetes. Insulin was not administered. During the test, urine was collected every 30min. for uALB determination. GFR was meassured by DTPA99Tc technique. Radioimnuneassays for uALB and glucagon determination. KAL and PRE-KAL stearase activity was meassured by substrate S2266 hydrolisis. Blood glucose concentration was 75.0±5.Qmg% in N and 197.0±21.0mg% in D. At 150 min., uALB in N, was 3784±0.87pg/min/1.73m2. The mean plus 3 D.S. was considered as the maximal normal valor: 12.00pg/min/1.73m2. In 13 D, uALB was 4.60±0.83μg/min/1.73m2 and was considered not having nephropathy. In 6 D, uALB was: 38.00±15.70μg/min/1.73m2 and considered as an expression of nephropathy. Urine collection of 24 hours did not show any difference in uALB between N and N. GFR was 101.0±8.3ml/min/1.73m2 in N and 144.0±8.0 in D (p<0.005). KAL and PRE-KAL were also higher in D that in N but no difference was seen in glucagon.
As conclusion: 1) the water load test is the most sensitive mean for detecting nephropathy in D patients; 2) no correlation was seen between uALB, KAL, PRE-KAL and GFR.
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Alvarado, C., Cresto, J., Voyer, L. et al. WATER OVERLOAD TEST FOR DETECTION OF DIABETES NEPHROPATHY, RELATION BETWEEN GLOMERULAR FILTRATION RATE (GFR), MICROALBUMINURIA (uALB), KALLIKREIN (KAL) AND GLUCAGON. Pediatr Res 26, 163 (1989). https://doi.org/10.1203/00006450-198908000-00029
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DOI: https://doi.org/10.1203/00006450-198908000-00029