Abstract
Exponentially growing cells of a AML cell line were separated by counterflow elutriation into subpopulations representing G0/G1, S and G2+M cells without disturbances of cellular metabolism. The separation was followed by DNA-flowcytometry. Specific binding of Insulin could be demonstrated with 20-25000 binding sites in G0/G1, 1000 - 2000 in S and 30-50000 in G2/M cells. The affinity of Insulin binding remained constant over the cell cycle. The specificity could be demonstrated by crosslinking experiments. Glucose transport was stimulated by Insulin independently of the cell cycle. In contrast gycogen synthesis could only be stimulated by Insulin in the G0/G1 phase. Insulin stimulated G1/S transit inducing proliferation and in addition the transit to G2 and through mitosis was accelerated. It could be demonstrated that these neoplastic cells remained under metabolic control of Insulin which has additionally proliferative action. The cells became independent from classical growth factors.
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Hartmann, W., Gola, R. & Vetter, U. 72 PROLIFERATIVE AND METABOLIC ACTION OF INSULIN IN A LEUKEMIC CELL LINE DURING THE CELL CYCLE. Pediatr Res 24, 272 (1988). https://doi.org/10.1203/00006450-198808000-00098
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DOI: https://doi.org/10.1203/00006450-198808000-00098