Abstract
Glibenclamide treatment of hypophysectomized rats augmented insulin, somatomedin, and skeletal growth. It is unknown if the sulfonylurea can directly enhance skeletal growth. Therefore chondrocytes from normal or hypophysectomized rats and from human nose septal cartilage were isolated and cultured in a semisolid medium for 14 days at 37°C with or without glibenclamide. Colony formation was determined. Results: Colony formation (20-50 colonies) without glibenclamide was designated as 100% (M±SEM).
Glibenclamide increased clonal growth. CelTs from hypox. rats formed less colonies than cells from normal rats. Therefore chondrocytes from human nose septal cartilage were cultured with and without an IGF I-receptor antibody ( IR-3, courtesy of Dr.S. Jacobs) in two dilutions: 1:50 and 1:1000. Glibenclamide (25-100 ng/ml) stimulated colony formation was completely blocked with both AK-dilutions, while in control experiments with 25 ng/ml IGF I, clonal growth was abolished with the IgF I-Ak dilution 1:50 but not 1:1000. Glibenclamide appears to be the first non-hormonal agent to augment growth of chondrocytes. The somatomedins may be involved in its action.
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Heinze, E., Vetter, U., Fussgänger, R. et al. THE SULFONYLUREA GLIBENCLAMIDE DIRECTLY STIMULATES GROWTH OF CHONDROCYTES. Pediatr Res 23, 112 (1988). https://doi.org/10.1203/00006450-198801000-00067
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DOI: https://doi.org/10.1203/00006450-198801000-00067