Abstract
The eucaryotic trifunctional protein (5, 10-methylenetetrahy-drofolate dehydrogenase -5, 10-methenyltetrahydrofolate cyclohy-drolase-10-formyltetrahydrofolate synthetase) catalyzes 3 consecutive steps in the interconversion of tetrahydrofolate derivatives; tetrahydrofolates are the one-carbon units required for the synthesis of thymidylate, purines and methionine. We have recently described the isolation of a human liver cDNA of 250 bp, coding for this protein, from a λgt11 expression library (Amer. J. Hum. Genet. 39: A204, 1986).
In this communication, we report the chromosomal mapping of the gene in somatic cell hybrids. Southern blot analysis of BamHI-digested DNA reveals 2 prominent bands in humans (∼19 and 14 kb) and one major band in Chinese hamsters (∼5 kb). In Chinese hamster X human hybrids, the 19 kb band is localized to human chromosome 14 (q21-qter) and tne 14 kb band, to the X chromosome. The assignment of the gene to separate loci suggests the presence of 2 homologous genes, or, more likely, the existence of a pseudogene. Ongoing studies with longer cDNA probes will enable us to determine the exact number of homologous genes in this family.
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Rozen, R., Hum, D., Barton, D. et al. CHROMOSOMAL ASSIGNMENT OF THE HUMAN GENE FOR THE TRIFUNCTIONAL FOLATE-DEPENDENT ENZYME: EVIDENCE FOR MULTIPLE LOCI. Pediatr Res 21 (Suppl 4), 294 (1987). https://doi.org/10.1203/00006450-198704010-00760
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DOI: https://doi.org/10.1203/00006450-198704010-00760