Abstract
Previous investigations have demonstrated that lactoferrin in human colostrum stimulates thymidine uptake into the DNA of harvested mature rat intestinal crypt cells. The effect of cow milk- and soy-based infant formulas on thymidine incorporation into DNA of rat intestinal crypt cells was studied. Crypt cells were harvested from adult male rats using the method of eversion and vibration. The cells were incubated in Trowell's T-8 medium with 10% fetal calf serum in a Dubnoff shaking water bath at 37°C gased with 95% O2 and 5% CO2. Each 50-ml incubation tube contained 4-5 × 105 cells in 1 ml of medium. When the skim fractions of the cow milk- and soy-based formulas were added to the incubated cells, 3H-thymidine incorporation into DNA was inhibited. This inhibition was reversed with the addition of human lactoferrin in amounts comparable to those found in human colostrum.
CONCLUSION: Cow milk- and soy-based infant formulas inhibit thymidine uptake into DNA of rat intestinal crypt cells. Addition of human lactoferrin to the formulas reverses the inhibition.
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Nichols, B., McKee, K. HUMAN LACIORERRIN FORTIFICATION OF FORMULAS PROMOTES THYMIDINE UPTAKE INTO DNA OF RAT INTESTINAL CRYPT CELLS. Pediatr Res 21 (Suppl 4), 273 (1987). https://doi.org/10.1203/00006450-198704010-00638
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DOI: https://doi.org/10.1203/00006450-198704010-00638