Abstract
Polyclonal antisera against rat visceral yolk sac (VYS) produces severe congenital malformations when injected into pregnant rats on the 9th day of gestation. To define and characterize the teratogen-stimulating antigen(s) from the VYS, monoclonal antibodies (MCA) against rat VYS were prepared. Spleen cells from BALB/c mice, hyperimmunized with VYS antigens purified by IEF and gel filtration were fused with two myeloma cell lines SP2/0-Ag 14 and P3x63 Ag. 8.653. Hybrids specific for VYS by indirect immunofluorescence were selected and cloned by limiting dilution and further expanded as ascitic tumor in BALB/c mice. More than 25 immunofluorescent hybrids for VYS have been selected and their biological activity defined. The first 12 clones were not teratogenic but since last year (1986) ascitic fluid from B-3 clone (IgG 2b type) induced CNS malformations and the clone D-4 (IgG 2a type) induced growtn retardations when injected into pregnant rats on the 9th day of gestation. Each teratogenic MCA stained the apical portion of VYS endodermal cell, tubular kidney brush border and failed to react with kidney glomeruli, Reicherts membrane, long, lens capsule and liver tissue by immunofluorescence. Both teratogenic MCA's failed to recognize the VYS antigens by Western blots. Further studies will be directed toward using the teratogenic MAC's to study the mechanism of teratogenesis and the quantitative aspects of embryonic nutrition during early organogenesis. (Supported by NIH)
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Jensen, M., Damjanov, I., Vega, P. et al. CONGENTIAL MALFORMATIONS INDUCED MY MONOCLONAL ANTI-BODIES AGAINST RAT VISCERAL YOLK SAC. Pediatr Res 21 (Suppl 4), 228 (1987). https://doi.org/10.1203/00006450-198704010-00371
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DOI: https://doi.org/10.1203/00006450-198704010-00371