Abstract
Immune reactions to hepatocyte membrane antigen has been suggested to play an important role in liver damage in several conditions. Antigens on the surface of cells are recognised in association with glycoproteins coded for MHC. In this study we have investigated expression of MHC class I & II antigens on isolated hepatocytes from 24 children with liver disease: 2 autoimmune chronic active hepatitis, 4 primary sclerosing cholangitis, 5 alpha-1 antitrypsin deficiency, 3 post-liver transplant, 3 hepatitis B related chronic liver disease, 7 minor histological changes. Hepatocytes were isolated from liver biopsies by mechanical disruption. Class I(HLA-A, B & C) antigens were detected by W6/32 monoclonal antibody(Mab) and Class II(HLA-DR) antigens by L243 Mab. Class I antigens were present on isolated hepatocytes in all cases while Class II were never detected. Positive stain for Class II was seen on Kuppfer cells. Expression of HLA-DR antigens on hepatocytes could be induced by a 72hr.incubation with 200 U of recombinant gamma interferon. These data suggest that immune recognition of hepatocyte membrane antigens may not be class II restricted. Alternatively, the HLA-DR positive Kuppfer cells might act as liver antigens presenting cells.
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Lobo-Yeo, A., Mieli-Vergani, G., Mowat, A. et al. 59. EXPRESSION OF CLASS I & CLASS II MAJOR HISTOCOMPATIBILITY COMPLEX)MHC) ANTIGENS ON HEPATOCYTES ISOLATED FROM CHILDREN WITH LIVER DISEASE. Pediatr Res 22, 106 (1987). https://doi.org/10.1203/00006450-198707000-00080
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DOI: https://doi.org/10.1203/00006450-198707000-00080