Abstract
Children with aCAH have impaired Concanavalin A(Con A) induced 'suppression of Pokeweed stimulated immunoglobulin synthesis. Since this system tests non-specific immunoglobulin production, we investigated suppression of in vitro production of antibody to Tetanus toxoid (TT), a T-dependent antigen, by lymphocytes from 11 children with aCAH and 14 age-matched healthy controls, all previously immunized. Both patients and controls had similar serum anti-TT levels (mean ± SEM 1.8 ± 0.85 lU/ml, 2.0 ± 0.86 IU/ml) and in vitro production of anti-TT in response to optimal dose of TT (0.005 IU/ml)(5.3 ± 0.88 × 10−3 IU/ml, 6.7 ± 0.94 × 10−3 IU/ml) as measured by ELISA. Suppressor function was induced by 24 hour incubation of lymphocytes with high dose TT (5 IU/ml) or with 20μg/ml Con A. Washed cells were then added to stimulated autologous lymphocytes. TT-induced suppression of anti-TT production was similar in patients (69.8 ± 4.2) and controls (72 ± 3.8%). In contrast, Con A-induced suppression of anti-TT was significantly lower in patients (15.7 ± 2.5%) than controls (46.7 ± 4.9% p<0.01 Rank Test). Our data indicate that antibody production to a T-cell-dependent antigen is under the control of at least two regulatory mechanisms, one antigen specific and one antigen non-specific, only the latter being defective in aCAH.
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Vegente, A., Lobo-Yeo, A., Alviggi, L. et al. ACTIVATION OF CYTOTOXIC-SUPPRESSOR HLA-DR POSITIVE T LYMPHOCYTES IN CHRONIC HEPATITIS B VIRUS (HBV) INFECTION IN CHILDHOOD. Pediatr Res 20, 698 (1986). https://doi.org/10.1203/00006450-198607000-00078
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DOI: https://doi.org/10.1203/00006450-198607000-00078