Abstract
We have demonstrated significant passive absorption of BS in proximal small intestine of the young rat (Ped Res 18:213A,1984). To evaluate the impact of carbohydrate absorption on the passive absorption of BS, young male rats were studied (n=15; age 38.6 ± 1.2 d; wt 142.2 ± 5.5 g - mean ± SEM). Each rat was anesthetized, the bile duct was cannulated and the (J) jejunum catheterized. G rats (n=8) had 1 ml of solution containing 0.25 to 10 mM taurocholate (TC), 3H-TC, Poly R-WS (non-absorbable marker), and 20mM d-glucose instilled into the J. M rats (n=7) received TC, tracers and 20mM d-mannitol. Bile was collected for 90 min. post-injection and counted. J and ileum were then divided into 8 segments, homogenized, and counted. Poly R-478 concentration per segment was used to calculate TC absorption. Data from previous rats given only TC (T) was compared to G and M data.
Poly R-478 recovery was 92.2 ± 2.1% and >92% of the marker was found in the proximal J (segs 1-3). The maximum rate of TC absorption (G-r=.98, p<.001(**); M-r=.96,**), the total TC absorbed (G-r=.95,**; M-r=.99,**) and the TC recovered in bile (G-r=.89, p<.01(*); M-r=.81, p<.05) correlated significantly with the administered TC dose in both G and M groups.
Passive TC absorption from proximal intestine in the presence of glucose is similar to that of TC alone - no consistent solute drag effect was seen. Decreased BS absorption in the presence of mannitol suggests that BS loss in malabsorptive diseases may relate to decreased passive BS absorption.
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Stahl, G., Fayer, J. & Watkins, J. 737 EFFECT OF INTRALUMINAL GLUCOSE (G) AND MANNITOL (M) ON THE PASSIVE ABSORPTION OF BILE SALTS (BS). Pediatr Res 19, 233 (1985). https://doi.org/10.1203/00006450-198504000-00767
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DOI: https://doi.org/10.1203/00006450-198504000-00767