Abstract
We examined NGF and EGF concentrations in synaptosomal fractions from mouse cerebral cortex during weaning. Pooled cortex tissues were homogenized in ice-cold 0.32 M sucrose with a glass-teflon homogenizer. The synaptosome enriched fraction was then prepared by differential centrifugation followed by a discontinuous sucrose density gradient. The purity of the preparation was confirmed by electron microscopy. Synaptosomal pellets were homogenized in phosphate buffered saline pH 7.4 and centrifuged to obtain 115,000 xg supernatant referred to as “synaptosomal extract (SE)”. βNGF and EGF concentrations were quantified by respective radioimmunoassay (RIA). The mean (± SEM) NGF and EGF concentration in whole brain (B), cerebral cortex (C) and SE were as follows:
Intraventricular injections of NGF and EGF 15 minutes prior to sacrifice did not significantly alter either NGF or EGF concentrations in SE.
Summary and conclusion: a) NGF and EGF are present in cerebral cortical synaptosomal fractions in high concentrations, b) their presence and differential distribution in brain suggest that both peptides may have physiological functions specific to synaptic regions.
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Weichsel, H., Lakshmanan, J., Kim, H. et al. 331 HIGH CONCENTRATIONS OF NERVE GROWTH FACTOR (NGF) AND EPIDERMAL GROWTH FACTOR (EGF) IN SYNAPTOSOMAL FRACTIONS OF MOUSE CEREBRAL CORTEX. Pediatr Res 19, 166 (1985). https://doi.org/10.1203/00006450-198504000-00361
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DOI: https://doi.org/10.1203/00006450-198504000-00361