Abstract
To investigate hormonal interactions in regulation of surfactant synthesis, we assayed PC synthesis in organ cultures of fetal rat (18 d), rabbit (23 d) and human (17-25 wk) lung maintained in serum-free Waymouth's medium for 2-6 d. Explants were exposed to no hormones, dexamethasone (Dex, 10-100 nM), T3 (2 nM), prolactin (PRL, 0.2-2 μg/ml) or combinations thereof. PC synthesis was assayed by incorporation of 3H-choline into PC for 4 h in 3-11 experiments. Results (mean±SE) are shown below.
In contrast to published reports for rat and human lung, we found no significant effect by PRL either alone or in the presence of other hormones. Results were equivalent for 2 sources of PRL (NIH & Sigma), a range of exposure times (1-6 d), a range of doses (0.001-8 μg/ml), incorporation rate of 3H-acetate into PC and distribution of cpm among phospholipids (rabbit), incorporation of choline into saturated PC (rat), and rocker vs lens paper/grid culture systems (human). We conclude that Dex and T3 have optimal effects on surfactant synthesis in cultured fetal lung in the absence of exogenous PRL.
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Ballard, P., Gonzales, L., Gross, I. et al. 233 INTERACTION OF GLUCOCORTICOID, THYROID HORMONE AND PROLACTIN IN STIMULATION OF PHOSPHATIDYLCHOLINE (PC) SYNTHESIS IN CULTURED FETAL LUNG. Pediatr Res 19, 149 (1985). https://doi.org/10.1203/00006450-198504000-00263
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DOI: https://doi.org/10.1203/00006450-198504000-00263