Abstract
Cord-blood IgE was determined prospectively in 300 neonates by an enzyme-immuno-assay(Phadezym-Prist)(EIA) and a radio-immuno-assay (Phadebas-Prist)(RIA). In order to obtain sufficient sensitivity for the EIA, determinations were performed with undiluted sera, and calibration curves were newly established. The lowest value determined was at 0.125 kU/l. A comparison of EIA and RIA revealed no significant differences for single determinations. The geometric mean was 0.246 kU/l; 2-SD was 1.0 kU/l for both test systems.
10% of the cord-blood values were above 1.0 kU/l (n=29 neonates); a follow-up of these 29 infants 18 months after birth detected 19 with symptoms of atopy (atopic dermatitis=14; allergic asthma=4; urticaria with cow-milk-allergy=1).
The presented data would suggest that EIA and RIA are comparably sensitive and valuable methods for the determination of IgE-levels in cord-blood. An early risk factor for the development of atopic diseases in infancy can thus be detected.
In addition, 200 of the 300 cord-blood sera were studied for T- and B-lymphocyte distribution by the use of monoclonal antisera in a routine immunofluorescence assay. A correlation between above normal values of IgE (> 1.0 kU/l) and the distinct lymphocyte populations, including T-helper- and T-suppressor-cells, could not be demonstrated.
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Bauer, C., Franz, R. & Belohradsky, B. CORD-BLOOD IgE DETERMINATION BY ENZYME-IMMUNO-ASSAY. CORRELATION BETWEEN ABOVE NORMAL IgE VALUES AND LYMPHOCYTE- POPULATIONS. Pediatr Res 19, 1119 (1985). https://doi.org/10.1203/00006450-198510000-00282
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DOI: https://doi.org/10.1203/00006450-198510000-00282