Abstract
The marked rise in intestinal absorption of calcium and in the placental transfer of this ion in rats during the last days of gestation is associated with an increase in CaBP 9K in the maternal duodenum and placenta (Delorme et al. J. Dev. Physiol. 1, 181, 1979). We now report a comparative analysis of the expression of the CaBP 9K gene in the maternal duodenum and in maternal and fetal parts of the placenta at different stages of the gestation in rat and also in specific tissues during the development of human fetuses. CaBP 9K mRNA levels were examined in these tissues by Dot-blot hybridization. Size analysis of the mRNA sequences was performed by electrophoretic fractionation on agarose gels and Northern hybridization to 32p cloned rat duodenal CaBP 9K cDNA. A homogeneous 500-600 nucleotide mRNA species was shown when RNA from different tissues was hybridized to the cDNA probe. In the rat, the highest concentration of CaBP 9K mRNA occured on the last day of gestation in both tissues. At this time, CaBP 9K mRNA was 20-30 times less concentrated in fetal and maternal parts of placenta than in the maternal duodenum. CaBP 9K mRNA concentration in the maternal duodenum doubled from day 15 to day 21 while in the maternal placenta CaBP 9K mRNA increased 7 fold during the same period. Studies in the human fetuses reveal that the CaBP 9K-Da mRNA appeared early during the development in specific tissues. This evolution of CaBP 9K mRNA levels in maternal duodenum and fetal placenta during pregnancy provides evidence for an active process of CaBP 9K biosynthesis associated with concomittant changes in calcium transfer in these tissues.
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Brun, P., Perret, C., De Maintenant, F. et al. EXPRESSION OF CHOLECALCIN (9,000MW CHOLECALCIFEROL-INDUCED CaBP) GENE DURING THE DEVELOPMENT OF RAT AND HUMAN FETUSES. Pediatr Res 19, 1116 (1985). https://doi.org/10.1203/00006450-198510000-00264
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DOI: https://doi.org/10.1203/00006450-198510000-00264