Abstract
5'-N-ethylcarboxamideadenosine (NECA) >2-chloroadenosine (2-C1A) >adenosine (A)> {(−)-N6-(R-phenyl-isopropyl)-adenosine } (−)-R-PIA > (+)-S-PIA, in μM concentrations, increase the intracellular level of adenosine 3',5'-monophosphate (cAMP) of human B and T (OKT4+ and OKT8+ phenotype) lymphocytes (L). Low concentrations of A (10−9-10−6 M) and (−)-R-PIA (10−9-10−6 M) inhibit the stimulating effect of PGE1, isoproterenol (ISO), histamine (H), NECA, cholera toxin (CT) and forskolin (FOK) on cAMP metabolism of L. This effect is inhibited by low doses of methylxanthines. 2',5'dideoxyadenosine (DDA) (10−6 - 2 × 10−4 M), a selective agonist of the P-site, and high concentrations of A (10−4-10−3 M) inhibit the effect of PGE1, ISO, H, CT and FOK in B and T L. The effect of DDA is not blocked by methylxanthines. These results support the hypothesis that human B and T (OKT4+ and OKT8+) L possess a membrane adenosine A2/Ra receptor. Human L also possess an inhibitory adenosine A1/Ri receptor and a P-site.
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Marone, G., Petracca, R., Vigorita, S. et al. ADENOSINE RECEPTORS ON HUMAN LYMPHOCYTES: 123. Pediatr Res 19, 764 (1985). https://doi.org/10.1203/00006450-198507000-00143
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DOI: https://doi.org/10.1203/00006450-198507000-00143