Abstract
The amount of S-adenosylhomocysteine in liver increases 2 to 3 fold during the first minute following death of the animal (Hoffman, D.R. et al. (1979) Can. J. Biochem. 57, 56-65), and postmortem increase in AdoHcy should be avoided when determining the physiological level of AdoHcy. Injection of mice with the drug combination ara-A plus dCF caused a massive increase in the AdoHcy content in several tissues of mice. A large fraction of the amount of AdoHcy in liver and to a lesser degree in kidney, heart and lung of mice so treated, could be attributed to a rapid postmortem accumulation of AdoHcy. This could be prevented by freezing the organs in vivo using liquid nitrogen.
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Helland, S., Ueland, P. DETERMINATION OF S-ADENOSYLHOMOCYSTEINE IN TISSUES FOLLOWING PHARMACOLOGICAL INHIBITION OF S-ADENOSYLHOMOCYSTEINE CATABOLISM: 84. Pediatr Res 19, 757 (1985). https://doi.org/10.1203/00006450-198507000-00104
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DOI: https://doi.org/10.1203/00006450-198507000-00104