Abstract
7-day old rats (15 g body weight) were injected subcutaneously with iodo-[1C] antipyrine (5 μ Ci). After a variable period, each pup was decapitated and arterial blood collected for scintillation counting. Brains were immediately removed and either prepared for isotopic counting or frozen for autoradiography. The brain: blood partition coefficient was determined (0.944 ± .066 ml/g (mean ± S.E.)). Both cerebral hemispheric blood flow (CHBF) and rCBF were calculated according to a formula derived from the Fick equation. CHBF equaled 66 ± 4 ml/100 g/min (mean ± S.E.), a value midway between reported 1-day old rat CBF and adult rat CBF. Auto-radiograms were of sufficient quality to permit microdensitometric readings of a minimum of 11 structures. rCBF ranged from 20 ml/100 g/min in subcortial white matter to 71 ml/100 g/min in the brain stem. Immature rat rCBF, as a proportion of adult rat rCBF, was greatest in brain stem.
Other 7 day old rats underwent right common carotid artery ligation and subsequent exposure to an 8% O2 environment. Right hemispheric flow was reduced to less than 5% of left hemispheric flow. This reduction was greatest in the distribution of the right middle cerebral artery. Vascular columns perpendicular to the pial surface, corresponding to columns seen metabolically and histopathologically, were not found. Thus, we have developed a relatively simple means of measuring rCBF in the immature rat and we have used it to study a model of cerebral hypoxia-ischemia.
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Lyons, D., Vannucci, R. & Reiter, E. 1442 REGIONAL CEREBRAL BLOOD FLOW (rCBF) IN THE IMMATURE RAT. Pediatr Res 19, 351 (1985). https://doi.org/10.1203/00006450-198504000-01466
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DOI: https://doi.org/10.1203/00006450-198504000-01466