Abstract
We describe the phenotypic and functional characteristics of an LBL derived from the bone marrow of a patient who died with an Epstein-Barr virus (EBV) related lymphoreticular malignancy 4 months after bone marrow transplantation for SCID. This LBL, designated DV-1, arose spontaneously 2–3 weeks after explantation and while initially dependent on autologous fibroblasts, subsequently has been maintained in standard suspension cultures. The B-cellorigin is suggested by the absence of T-cell markers and the presence of DR and Blantigen on 80–90%, surface IgM on 20–30%, and IgD on 20–30% of the cells. The predominant light chain type is κ (72%) with a small percentage of cells expressing λ. The cells show a norma 146XY karyotype and the host's HLA phenotype. Greater than 90% of the cells are positive for EBV nuclear antigen and show the typical appearance and growth characteristics of EBV transformed LBL. Phytohemagglutinin inhibited (12–39%), pokeweed mitogen had no effect on, goat anti-μ chain antiserum stimulated (57%), and staphylococcus aureus Cowan strain A inhibited (20–80%) thymidine incorporation by DV-l in a dose related manner. These characteristics are similar to those described for other EBV derived LBL's reported in the literature and to an independently derived LBL (LA350) to which we compared DV-1. This line holds useful potential for future functional and morphological studies on B-cell lines derived from immunodeficient patients.
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Rosenblatt, H., Ritz, J., Parikh, N. et al. 1022 A LYMPHOBLASTOID B-CELL LINE (LBL) DERIVED FROM A 12-YEAR-OLD BOY WITH SEVERE COMBINED IMMUNODEFICIENCY (SCID). Pediatr Res 19, 281 (1985). https://doi.org/10.1203/00006450-198504000-01052
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DOI: https://doi.org/10.1203/00006450-198504000-01052