Abstract
Monoclonal antibody 31D8 binds strongly to the major subpopulation of neutrophils (PMN) that depolarize and respond chemotactically to formy1 peptide (FMLP). 31D8 antigen appears by the myelocyte stage of differentiation (Clin. Res. 32:558A. 1984). Newborns have decreased PMN chemotaxis compared to adults. We compared 31D8 binding to PMN from 9 healthy neonates and adults using indirect fluorescent labeling and flow cytometry. All fluorescence curves generated were negatively skewed and some had a less fluorescent peak on the skewed side of the major peak. In order to determine functional significance of the labeling pattern, PMN were placed in chemotactic chambers with FMLP. Mean chemotactic values were 43.5 ± 2.μm for neonate and 64.3 ± 12.4 μrn for adult (p < 0.05). Responsive cells which traversed filters into bottom chambers were collected and labeled with 31D8. These responsive PMN were contained within a symmetric curve about the major peak (31D8 “bright”) of the unseparated population and did not include PMN which contributed to the negative skew (31D8 “dull”). This observation allowed calculation of the size of the 31D8 “bright” and “dull” subpopulations. Adult PMN consisted of 79.2 ± 5.8% 31D8 “bright” cells while neonate PMN consisted of 67.5 ± 4.5% 31D8 “bright” cells (p < 0.0002). These data suggest that neonate PMN are less responsive chemotactically than adult PMN in part because neonate PMN include a larger percentage of less responsive 31D8 “dull” cells.
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Krause, P., Kosciol, C., Pontius, L. et al. 1001 NEUTROPHIL HETEROGENEITY IN NEONATES AND ADULTS. Pediatr Res 19, 277 (1985). https://doi.org/10.1203/00006450-198504000-01031
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DOI: https://doi.org/10.1203/00006450-198504000-01031