Abstract
The antibody 3F8, an IgG3 murine Mab we have developed to human NB, could specifically target iodine 131 to human NB xeno-xenograft with tumor to non tumor ratios of 10–100 and a relative radiation dose deposition to normal organs of 1 to 20% of that to the tumor. We therefore studied its efficacy in tumor therapy. Nude mice with actively growing so human NB (1–2 gm size) were injected with 0.125 to 1 mCi iodine carried on 100 to 200 ug Mab 3P8. Tumor size was followed by direct measurement. Actual tumor weight and measured size showed good correlation (r= 0.985). Tumor radioactivity over time was calculated from the gamma images using a known 131–I standard. All the tumors had effective half lives averaging 48 hours. Radiation dose to individual tumors were calculated. Tumor shrinkage only occurred with 131–1 3F8 Mab, but not with nonradioactive 3F8 or radiolabeled irrelevant Mab. While control mice tumor enlarged by 10 fold, treated tumor showed 95% shrinkage by 12 days. Both the rate of shrinkage and duration of response were dose dependent. Only those tumors that received 4700 rads were completely ablated without recurrence. There were no toxicities except reversible weight loss. Thus, human NB xenografts could be effectively eradicated using iodine 131 labeled Mab 3F8 with tolerable toxicities.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cheung, NK., Landmeier, B., Ellery, S. et al. 963 COMPLETE TUMOR ABLATION WITH IODINE 131 RADIOLABELED MONOCLONAL ANTIBODY (Mab) AGAINST HUMAN NEUROBLASTOMA (NB) XENOGRAFTED IN NUDE MICE. Pediatr Res 19, 271 (1985). https://doi.org/10.1203/00006450-198504000-00993
Issue Date:
DOI: https://doi.org/10.1203/00006450-198504000-00993