Abstract
Helper/suppressor (H-S) cell abnormalities are found in more than 70% of hemophilia persons who are receiving factor VIII concentrates. Interleukin 2 (IL2) or T cell growth factor is a lymphokine produced by the helper cell as well as other cells. We studied 12 hemophilia subjects, 7 who received factor VIII concentrates and 5 who received cryoprecipitate. All those receiving factor VIII and 2 of the 5 receiving cryoprecipitate had reversed H-S ratios. IL2 was assayed on the supernates of PHA-stimulated mononuclear cells previously isolated on a ficoll-hypaque density gradient. The target cell was a human IL2 dependent T cell line established by stimulating normal mononuclear cells with PHA for 3 days. The cells were incubated for a further 4 days in media containing 50%, 4x concentrated IL2 previously prepared from PHA stimulated supernates pooled from normals.
Significant high levels of IL2 were found in hemophilia persons receiving factor VIII concentrate and cryoprecipitate. It is possible that a receptor defect accounts for the H cell failure.
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Katz, J., Walter, B., Bennetts, G. et al. 921 HIGH LEVELS OF INTEKLEUKIN 2 IN HEMOPHILIA PERSONS WITH ABNORMAL HELPER-SUPPRESSOR CELL NUMBERS. Pediatr Res 19, 264 (1985). https://doi.org/10.1203/00006450-198504000-00951
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DOI: https://doi.org/10.1203/00006450-198504000-00951