Abstract
Neonates with necrotizing enterocolitis have a peritoneal inflammatory response which has not been examined as a factor in the neutropenia of NEC. The rat peritoneum was used to determine the independent effects of protein and bacterial toxin on neutrophil mobilization.
30 juvenile rats were divided into 3 groups. Casein(10 mg), cholera enterotoxln(lug) or saline were injected IP in 3 trials. In 2 trials rats were sacrificed at 5 hrs., and in the 3rd at 24 hrs. Peripheral blood(B) peritoneal(P) and bone marrow(B.M.) total WBC counts with differentials were obtained. The distribution of mature neutrophils (polys and bands) in each compartment was determined.
Casein and toxin provoke mobilization of mature neutrophils into the peritoneum. With toxin there is also a fluid response which at 24 hrs was exudative precluding quantitation of cells. Although in 2 experiments bone marrow neutrophils were depleted, stores were adequate to avoid neutropenia. This may not be the case in neonates with NEC. Dietary protein (casein) and bacterial toxin in the peritoneum of babies with NEC may exert a neutrophil chemotactic response.
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Balcom, R., Clark, D. & Rokahr, J. NEUTROPHIL RESPONSE TO CHEMOTAXINS IN THE RAT PERITONEUM: A MODEL FOR THE INFLAMMATORY RESPONSE IN NEC. Pediatr Res 18 (Suppl 4), 309 (1984). https://doi.org/10.1203/00006450-198404001-01297
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DOI: https://doi.org/10.1203/00006450-198404001-01297