Abstract
Prostaglandin (PG) synthesis by rabbit alveolar macrophages (Mø) was reported to be stimulated by heterologous , homologous and, autologous sera. In the present study we stimulated alveolar Mø prelabelled with 14C-arachidonic acid with variuos serum preparations, complement proteins and quantitated their PG release by thin layer chromatography. Our experiments showed that: 1) trypsinized rabbit serum(RS), RS activated with aggregated IgG and zymosan, stimulated PG release by Møs. 2) Activation of RS with zymosan was more efficient stimulating PG synthesis than RS activated with aggregated IgG. 3) When C4 deficient guinea pig serum was used, it was clear that activation of the alternative pathway of complement (APC) independent of the classical pathway, stimulated PG release. 4) We were able to abolish the stimulatory effect of serum and activated C3 (but not zymosan) by blocking the Mø C3b receptor (c3bR) with anti-C3bR preincubation. Thus we concluded that activation of the alternative pathway of complement plays an important role in PG synthesis by alveolar macrophages.
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Arroyave, C., Hsueh, W. & Jordan, P. PROSTAGLANDIN SYNTHESIS BY RABBIT ALVEOLAR MACROPH GES MEDIATED BY THE ALTERNATIVE COMPLEMENT PATHWAY ACTIVATION. Pediatr Res 18 (Suppl 4), 252 (1984). https://doi.org/10.1203/00006450-198404001-00955
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DOI: https://doi.org/10.1203/00006450-198404001-00955