Abstract
Cyanide insensitive O2 consumption in zymosan stimulated platelets.
We investigated the O2 consumption of human platelets, stimulated by zymosan,an inducer of release reaction(Zucker and Grant, 1974).The platelet suspensions(in PBS, pH 7.4) were prepared from fresh blood samples,by separating the leukocytes from PRP (contamination < 1 cell/108 platelets) through a layer of Ficoll (23% w/v). Zymosan was incubated with autologous citrated plasma for 30 min and then washed three times in saline. The O2 consumption was measured polarographically by a Clark electrode. The KCN ( 1mM) treated platelets, after addition of NADH (1mM) showed a O2 uptake of 1±0.3 nmol/min/109 platelets even in the presence of ASA (100mM) and TYA(225μg/ml) as the mean ± SD of 15 experiments.There was no increased O2 consumption in non opsonized zymosan stimulated control platelets.The reaction failed to occur by zymosan incubated with hydrazine treated plasma.Our results suggest that an activation of a NADH CN-insensitive oxidase by an immunological stimulus,occurs in platelets like that polimorphonuclear leukocytes.
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Del, P., Menichelli, A., Mancuso, G. et al. Cyanide insensitive O2 consumption in zymosan stimulated platelets. Pediatr Res 14, 1429 (1980). https://doi.org/10.1203/00006450-198012000-00125
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DOI: https://doi.org/10.1203/00006450-198012000-00125