Abstract
The protective effect of Poly I:C during sisomicin nephrotoxicity in rabbits was determined by measurement of the urinary enzymes alkaline phosphatase (AF),lactic dehydrogenase (LDH) and N-a-cetyl-β-glycosaminidase(NAG). 18 rabbits were divided in 3 Groups. Separate 24 hours urines were assayed for controls(AF=110±20SD, LDH=25±4SD,NAG=30±6SD).Group 1,received a subcutaneous dose of sisomicin,200mg/kg,Group 2,a subcutaneous dose of Poly I:C,5mg/kg followed 24 hours later by sisomicin and Group 3,only Poly I:C. 24 hours urines were collected for the next 5 days and assayed for enzymes.Fractional sodium excretion(%fENa)was calculated on the 1st and 6th day.Urinalysis was done daily.All rabbits were killed on the 6th day and the kidneys were examined by light microscopy.Group 1,presented a sharp rise in the urinary enzymes (AF=825±95SD,LDH=164±18SD,NAG=650±76SD),Group 2,a gradual rise in the urinary enzymes with the peak on the 5th day(AF=655±92SD,LDH =112±14SD,NAG=480±54SD)and Group 3,only a slight elevation(AF=156±24SD,LDH=37±6SD,NAG=60±4SD).Only in Group 1,the %fENa was 7.5-9.6% on the 5th day(controls:0.62-0.85%),the urinalysis showed hyaline casts and red cells and the kidneys' light microscopy revealed the proximal tubular cells swollen with fine vacuolation of the cytoplasm.The results support the view that Poly I:C,a toxic substance itself,protects the kidney cells from damage,most probably,by induction of interferon production.
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Zeis, P., Nacopoulou, L., Gouriotis, D. et al. PROTECTIVE EFFECT OF POLY I:C AGAINST SISOMICIN NEPHBOTOXICITY IN RABBITS. Pediatr Res 14, 1007 (1980). https://doi.org/10.1203/00006450-198008000-00202
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DOI: https://doi.org/10.1203/00006450-198008000-00202