Abstract
We investigated the possibility of labeling platelets by stable tracers. The concentration of the tracers was estimated by the Fluorescence X radioisotopic technique using a 3mCi Cd-109 source, a Xe-filled proportional detector and a single channel analyzer. In this paper we compared the in vitro survival of human platelets, anticoagulated with EDTA, stored at 22°C, labeled with stable Rb, with that of cells marked with 51Cr. Three experiments were performed. A typical curve is shown. A similar investigation was performed in vivo in rabbits. The mean half-life deduced from ten animals was 22±3h (SE), for Rb labeled platelets; 18±2h for 51Cr marked platelets. The elution rate of the stable tracer shows no difference in comparison with that of 51Cr.Rb as a tracer offers several advantages (low toxicity, no exposure to radiation) and it can represent a valuable alternative to radioisotopes in the study of the platelet survival for the pediatric research.
Article PDF
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Principe, D., Cesareo, R., Tallarida, B. et al. Labeling blood platelets with stable tracers.: 95. Pediatr Res 14, 180 (1980). https://doi.org/10.1203/00006450-198002000-00122
Issue Date:
DOI: https://doi.org/10.1203/00006450-198002000-00122